QPSOBT: One codon usage optimization software for protein heterologous expression

Key Laboratory of Industrial Biotechnology, School of Biotechnology, JiangNan University, 214122, Wuxi, Jiangsu, China
Journal of Bioinformatics and Sequence Analysis 07/2010; 2:25-29.

ABSTRACT QPSOBT is a codon usage optimization software based on the Quantum-behaved Particle Swarm Optimization (QPSO) algorithm. It can design synthetic genes of multikilobase sequences for protein heterologous expression rapidly. The program runs on .NET platform. Compared to the existing codon optimization software and web services, QPSOBT is able to generate better results when DNA/RNA sequence length is less than 6 kb which is a commonly-used range, especially when some restriction sites need to be removed. QPSOBT is freely available (

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