Microwave-assisted synthesis of biofunctional and fluorescent silicon nanoparticles using proteins as hydrophilic ligands.

Institute of Functional Nano & Soft Materials, FUNSOM and Jiangsu Key Laboratory for Carbon-based Functional Materials & Devices, Soochow University, Suzhou 215123, China.
Angewandte Chemie International Edition (Impact Factor: 11.34). 07/2012; 51(34):8485-9. DOI: 10.1002/anie.201202085
Source: PubMed

ABSTRACT Protective shell: A microwave-assisted method allows rapid production of biofunctional and fluorescent silicon nanoparticles (SiNPs), which can be used for cell labeling. Such SiNPs feature excellent aqueous dispersibility, are strongly fluorescent, storable, photostable, stable at different pH values, and biocompatible. The method opens new avenues for designing multifunctional SiNPs and related silicon nanostructures.

  • [Show abstract] [Hide abstract]
    ABSTRACT: Near-infrared (NIR, 700-900 nm) fluorescent quantum dots are highly promising as NIR bioprobes for high-resolution and high-sensitivity bioimaging applications. In this article, we present a class of NIR-emitting CdTe/CdS/ZnS core-shell-shell quantum dots (QDs), which are directly prepared in aqueous phase via a facile microwave synthesis. Significantly, the prepared NIR-emitting QDs possess excellent aqueous dispersibility, strong photoluminescence, favorable biocompatibility, robust storage-, chemical-, and photo-stability, and finely tunable emission in the NIR range (700-800 nm). The QDs are readily functionalized with antibodies for use in immunofluorescent bioimaging, yielding highly spectrally and spatially resolved emission for in vitro and in vivo imaging. In comparison to the large size of 15-30 nm of the conventional NIR QDs, the extremely small size (∼4.2 nm or 7.5 nm measured by TEM or DLS, respectively) of our QDs offers great opportunities for high-efficiency and high-sensitivity targeted imaging in cells and animals.
    Biomaterials 09/2013; · 8.31 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: During the past ten years significant advances have been achieved in quantum dot (QD) research field. The new synthetic methods and the discovery of new types of QDs have enabled a variety of new applications of QDs for bioimaging and biosensing. This review will focus on the most recent progress of QDs for biomedical applications. Ample examples will be given in this review on newly developed synthetic methods of QDs, non-toxic QDs, QDs for biomolecule detection, cell and animal imaging, and disease therapy.
    Colloids and surfaces B: Biointerfaces 01/2014; · 4.28 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Multidrug resistance (MDR) remains a major challenge for cancer treatment thus far. Free doxorubicin (DOX, one of the most widely used chemotherapy agents for cancer treatment) generally features a large value of resistant factor (RF), which is regarded as a significant parameter to assess therapeutic efficiency of cross-resistance. To address this issue, we herein present a kind of silicon nanowires (SiNWs)-based drug nanocarriers (SiNW-DOX), which is high-efficacy for treatment of drug-resistant cancer cells. Typically, drug-resistance cancer cells (e.g., MCF-7/ADR cells) can be significantly inhibited by the SiNWs-based nanocarriers, exhibiting ∼10% cell viability during 72-h incubation with the SiNWs-DOX (80 μg mL−1 DOX), which is in sharp contrast to free DOX-treated cells preserving ∼40% cell viability. Remarkably, the RF value of SiNW-DOX is as low as ∼2.0, which is much better than that (∼300) of free DOX under the same experiment conditions. To the best of our knowledge, it is the lowest RF value ever reported by nanomaterials-based drug carriers (3.3–24.7).
    Biomaterials 01/2014; 35(19):5188–5195. · 8.31 Impact Factor