Transmission of Mycobacterium avium subsp. paratuberculosis: Dose-response and age-based susceptibility in a sheep model.

Faculty of Veterinary Science, The University of Sydney, 425 Werombi Road, Camden, 2570 NSW, Australia.
Preventive Veterinary Medicine (Impact Factor: 2.51). 07/2012; 107(1-2):76-84. DOI: 10.1016/j.prevetmed.2012.05.014
Source: PubMed

ABSTRACT Factors which influence the transmission of Mycobacterium avium subsp. paratuberculosis (MAP) between susceptible hosts are poorly defined, despite this organism causing economically significant disease in ruminants worldwide. A randomised longitudinal field trial was conducted using natural pasture-based exposure of 840 Merino sheep in a factorial design to test infection and disease outcomes in relation to age at first exposure and the level of exposure to MAP. Pasture contamination was initiated by MAP infected "donor" sheep which were present for 14.5 weeks of the 2.5 year study period. Sheep exposed to higher doses had 3.5 times greater odds to shed MAP in their faeces (assessed by faecal culture) compared to animals exposed to lower doses of infection. Similarly, sheep exposed to MAP as lambs had 7 times higher odds to shed MAP compared to sheep that were exposed for the first time as adults. However, animals of all ages and exposed at all doses were equally likely to be colonised by MAP (measured by culture of intestinal tissues) suggesting that some animals are inherently susceptible to infection. Age at first exposure was a significant determinant of histopathological lesion development, as lambs had about three times higher odds of developing severe lesions than adults after equivalent time (P=0.026). Mortalities due to paratuberculosis were strongly determined by the level of exposure; sheep exposed to high doses had 18 fold higher odds of death (P=0.007). Sheep exposed as lambs had 5 fold higher odds of dying due to paratuberculosis than adults (P=0.046). The results of this study provide sound experimental evidence for management recommendations in extensively grazed livestock to reduce the transmission of MAP by limiting exposure of young animals and reducing the levels of MAP pasture contamination.

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Johne's disease (JD) is a chronic enteric disease caused by Mycobacterium avium subsp. paratuberculosis that affects ruminants. Transmission is by the faecal-oral route. A commonly used ante-mortem diagnostic test for the detection of M. avium subsp. paratuberculosis in faeces is liquid culture, however a major constraint is the 2-3 months incubation period. Rapid detection methods for M. avium subsp. paratuberculosis based on PCR have been reported but comprehensive validation data are lacking. We describe a new test, High-Throughput-Johnes (HT-J), to detect M. avium subsp. paratuberculosis in faeces. Diagnostic accuracy was compared with liquid radiometric (BACTEC) faecal culture using samples from cattle (n=1330; 23 herds) and sheep (n=596; 16 flocks). The multistage protocol involves recovery of M. avium subsp. paratuberculosis cells from a faecal suspension, cell rupture by bead beating, extraction of DNA using magnetic beads and IS900 quantitative PCR. The limit of detection of the assay was 0.0005 pg and limit of quantification was 0.005 pg M. avium subsp. paratuberculosis genomic DNA. Only M. avium subsp. paratuberculosis was detected from a panel of 51 mycobacterial isolates, including 10 with IS900-like sequences. Of the 549 culture negative faecal samples from unexposed herds and flocks, 99% were negative in the HT-J test while 60% of bovine and 84% of ovine culture positive samples were positive in the HT-J test. As similar total numbers of samples from M. avium subsp. paratuberculosis-exposed animals were positive in culture and HT-J tests in both species, and as a McNemar's test was not significant, these methods probably have similar sensitivity, but the true diagnostic sensitivity of either test is unknown. These validation data meet consensus-based reporting standards for diagnostic test accuracy studies for paratuberculosis and Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines. The HT-J assay has been approved for use in JD control programs in Australia and New Zealand.
    Journal of clinical microbiology 12/2013; · 4.23 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Abstract This review addresses the survival and persistence of Mycobacterium avium subsp. paratuberculosis (MAP), the causative pathogen of Johne's disease (JD), once it has left its ruminant host. JD has significant economic impact on dairy, beef and sheep industries and is difficult to control due to the long-term sub-clinical nature of the infection, intermittent or persistent MAP shedding during and after this period, inadequate test effectiveness, and the potential for MAP to exist for extended periods outside the host. The role that environmental factors play in the persistence and spread of MAP and consequent disease is assessed. Published risk factor analysis, organism survival across various environmental media and conditions, presence and spread in ruminant and non-ruminant wildlife, and the general potential for survival and multiplication of MAP ex-host both on and off-farm are discussed and knowledge gaps highlighted. An inclusive approach to disease management that takes into account the persistence and transport of the causative organism in on-farm soils and waters, land use and management, dispersal by domestic and non-domestic host species, as well as general animal husbandry is required on those farms where more traditional approaches to disease management have failed to reduce disease prevalence.
    Critical Reviews in Microbiology 03/2014; · 6.09 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Diagnostic tests used for Johne's disease in sheep either have poor sensitivity and specificity or only detect disease in later stages of infection. Predicting which of the infected sheep are likely to become infectious later in life is currently not feasible and continues to be a major hindrance in disease control. We conducted this longitudinal study to investigate if a suite of diagnostic tests conducted in Mycobacterium avium subspecies paratuberculosis (MAP) exposed lambs at 4 months post infection can accurately predict their clinical status at 12 months post infection. We tracked cellular and humoral responses and quantity of MAP shedding for up to 12 months post challenge in 20 controls and 37 exposed sheep. Infection was defined at necropsy by tissue culture and disease spectrum by lesion type. Data were analysed using univariable and multivariable logistic regression models and a subset of variables from the earliest period post inoculation (4 months) was selected for predicting disease outcomes later on (12 months). Sensitivity and specificity of tests and their combinations in series and parallel were determined. Early elevation in faecal MAP DNA quantity and a lower interferon gamma (IFNγ) response were significantly associated with sheep becoming infectious as well as progressing to severe disease. Conversely, early low faecal MAP DNA and higher interleukin-10 responses were significantly associated with an exposed animal developing protective immunity. Combination of early elevated faecal MAP DNA or lower IFNγ response had the highest sensitivity (75%) and specificity (81%) for identifying sheep that would become infectious. Collectively, these results highlight the potential for combined test interpretation to aid in the early prediction of sheep susceptibility to MAP infection.
    Preventive Veterinary Medicine 08/2013; · 2.51 Impact Factor