Article

Experimental study on the treatment of rabbit corneal melting after alkali burn with Collagen cross-linking.

Ophthalmic Center, No.474 Hospital of Chinese PLA, Urumchi 830013, Xinjiang Uygur Autonomous Region, China.
International journal of ophthalmology 01/2012; 5(2):147-50. DOI:10.3980/j.issn.2222-3959.2012.02.06 pp.147-50
Source: PubMed

ABSTRACT To evaluate the effect of Collagen cross-linking on the prevention of melting in rabbit corneas after alkali burn.
Twenty New Zealand white rabbits were randomly divided into model control group and collagen cross-linking treatment group. The second group of rabbits received collagen cross linked treatment. Both groups were applied with antibiotic eye drops to prevent infection. The corneas were evaluated for melting, opacity, pathological and immunohistochemistry, record the changes when 28 days after the animals were killed.
In the control group, 6 out of 8 rabbits showed corneal melting after injury (14±4) days, while two corneal perforated. In collagen cross-linking treatment group, one rabbit showed corneal melting after injury 23 days, without corneal perforation; corneal dissolution rate between the two groups was significantly different (P <0.05). Pathological examination suggested that in the treatment group, mild corneal edema, mild damage to collagen fibers, inflammatory cell infiltration was significantly less than the control group. Immunohistochemistry showed that corneal collagen fibers arranged in neat rows in the control group.
Collagen cross-linking treatment not only can prevent and delay the corneal melting after alkali burn, but also can reduce the destruction of corneal collagen fibers and infiltration of inflammatory cells in the corneal tissue.

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  • Article: Potential use of riboflavin/UVA cross-linking in bullous keratopathy.
    Gregor Wollensak, Henning Aurich, Christopher Wirbelauer, Duy-Thoai Pham
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    ABSTRACT: Collagen cross-linking of the cornea has been shown by us to have an antiedematous effect in the cornea. The aim of the present study was to examine if this effect can be used for the treatment of bullous keratopathy. This clinical interventional case series included 3 patients (3 eyes) with bullous keratopathy due to pseudophakia, corneal transplant rejection, and Fuchs' endothelial dystrophy. After dehydration for 1 day using 40% glucose, the central 8 mm of the cornea were abraded and cross-linked with the photosensitizer riboflavin and UVA (370 nm, 3 mW/cm(2)) for 30 min. Optical coherence tomography pachymetry measurements of the central cornea were performed at various time intervals. Corneal thickness was reduced by 90.33 +/- 17.04 microm on average 3 days after cross-linking and by 93.67 +/- 14.22 microm after 8 months. The bullous changes of the epithelium were markedly improved, resulting in loss of pain and discomfort. Visual acuity was significantly improved in the case without prior stromal scarring. Cross-linking might become another useful tool in the treatment of bullous keratopathy. It is primarily suited for patients with pain symptoms, restricted visual prognosis or to extend the time interval for an upcoming corneal transplantation.
    Ophthalmic Research 01/2009; 41(2):114-7. · 1.56 Impact Factor
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Keywords

8 rabbits
 
antibiotic eye
 
Collagen cross-linking
 
Collagen cross-linking treatment
 
collagen cross-linking treatment group
 
control group
 
corneal collagen fibers
 
corneal dissolution rate
 
corneal tissue
 
inflammatory cell infiltration
 
inflammatory cells
 
injury 23 days
 
mild corneal edema
 
model control group
 
New Zealand white rabbits
 
P <0.05). Pathological examination
 
rabbit corneas
 
second group
 
treatment group
 
two groups