Differential distribution of ABCB1 and ABCC1 transporters in sea urchin pluteus larvae.

Departamento de Microbiologia, Centro de Pesquisa Aggeu Magalhães, FIOCRUZ, Recife-PE, Brazil.
Molecular Reproduction and Development (Impact Factor: 2.81). 06/2012; 79(8):501. DOI: 10.1002/mrd.22065
Source: PubMed

ABSTRACT ABC transporters are expressed in eukaryotic and prokaryotic cell membranes, and are involved in the transport of ions and several endogenous or exogenous compounds. Intracellular calcein accumulation assays associated with ABC transporter blockers have been used to evaluate ABC proteins activity in several cell types. Here we show a notable differential distribution pattern of ABCB1 and ABCC1 transporter activity in sea urchin pluteus larvae. Mol. Reprod. Dev. © 2012 Wiley Periodicals, Inc.

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    ABSTRACT: Embryos of marine organisms whose development occurs externally are particularly sensitive to ultraviolet (UV) light (bands A and B, respectively UVA and UVB). ATP-binding cassette (ABC) transporters are the first line of cellular defense against chemical or physical stress. The present work investigated the involvement of ABC transporters on UVA or UVB effects on eggs, spermatozoa, and embryonic cells of the sea urchin Echinometra lucunter. Gametes or embryos were exposed to UVA (3.6 to 14.4 kJ m(-2) ) or UVB (0.112 to 14.4 kJ m(-2) ), and embryonic development was monitored by optical microscopy at different developmental stages in the presence or absence of the ABC-transporter blockers reversin205 (ABCB1 blocker) or MK571 (ABCC1 blocker). Eggs, spermatozoa, and E. lucunter embryos were resistant to UVA exposure. Resistance to the harmful effects of UVB was strongly associated to ABC transporter activity (embryos > eggs > spermatozoa). ABCB1 or ABCC1 blockage promotes the injurious effects of UVA on spermatozoa. ABCC1 transporter blockage increased UVB-dependent damage in eggs while ABCB1 transporter inhibition increased harmful effects of UVB in embryonic cells. ABC-transporter activity was not, however, affected by UVB exposure. In conclusion, the present study is the first report about the protective role of ABC transporters against the harmful effects of UVA and UVB on sea urchin eggs and embryonic cells. Mol. Reprod. Dev. © 2013 Wiley Periodicals, Inc.
    Molecular Reproduction and Development 11/2013; · 2.81 Impact Factor

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