Bacitracin-Conjugated Superparamagnetic Iron Oxide Nanoparticles: Synthesis, Characterization and Antibacterial Activity
ABSTRACT Bacitracin-conjugated superparamagnetic iron oxide (Fe(3) O(4) ) nanoparticles were prepared by click chemistry and their antibacterial activity was investigated. After functionalization with hydrophilic and biocompatible poly(acrylic acid), water-soluble Fe(3) O(4) nanoparticles were obtained. Propargylated Fe(3) O(4) nanoparticles were then synthesized by carbodiimide reaction of propargylamine with the carboxyl groups on the surface of the iron oxide nanoparticles. By further reaction with N(3) -bacitracin in a Cu(I) -catalyzed azide-alkyne cycloaddition, the magnetic Fe(3) O(4) nanoparticles were modified with the peptide bacitracin. The functionalized magnetic nanoparticles were characterized by powder X-ray diffraction, X-ray photoelectron spectroscopy, TEM, zeta-potential analysis, FTIR spectroscopy and vibrating-sample magnetometry. Cell cytotoxicity tests indicate that bacitracin-conjugated Fe(3) O(4) nanoparticles show very low cytotoxicity to human fibroblast cells, even at relatively high concentrations. In view of the antibacterial activity of bacitracin, the biofunctionalized Fe(3) O(4) nanoparticles exhibit an antibacterial effect against both Gram-positive and Gram-negative organisms, which is even higher than that of bacitracin itself. The enhanced antibacterial activity of the magnetic nanocomposites allows the dosage and the side effects of the antibiotic to be reduced. Due to the antibacterial effect and magnetism, the bacitracin-functionalized magnetic nanoparticles have potential application in magnetic-targeting biomedical applications.
- SourceAvailable from: Anselmo Jesús Otero-González
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ABSTRACT: New magnetic core shell nanoparticles were synthesized consisting of magnetite cores and poly-(O-propargyl acrylate) shells. Strong fixing of the shells was achieved by primary anchoring phosphates or α-dihydroxydiphosphonates containing acrylate or methacrylate functionalities. The magnetic nanoparticles are attractive as supports for a variety of function which can be easily introduced by Cu-catalyzed alkyne azide cycloaddition (CuAAC, a click reaction). In this way, also the loading of the magnetic nanoparticles with propargyl units was determined by reaction with 4-azidoacetophenone and analysis of the supernatant. In order to demonstrate the attractiveness of the magnetic nanoparticles a novel azido-containing conjugate with biotin as recognition function and dansyl as fluorescence marker was introduced by CuAAC reaction. All NP show superparamagnetic behavior with high-saturation magnetization values and were further characterized by FTIR, photoelectron spectroscopy and TEM.Journal of Nanoparticle Research 06/2013; 15(6). DOI:10.1007/s11051-013-1747-6
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ABSTRACT: Superparamagnetic iron oxide nanoparticles (SPIONs) have shown great promise in biomedical applications. In this review, we summarize the recent advances in the design and fabrication of core-shell and hetero-structured SPIONs and further outline some exciting developments and progresses of these multifunctional SPIONs for diagnosis, multimodality imaging, therapy, and biophotonics.Nanoscale 07/2013; 5(17). DOI:10.1039/c3nr01616a