Bacitracin-conjugated superparamagnetic iron oxide (Fe(3) O(4) ) nanoparticles were prepared by click chemistry and their antibacterial activity was investigated. After functionalization with hydrophilic and biocompatible poly(acrylic acid), water-soluble Fe(3) O(4) nanoparticles were obtained. Propargylated Fe(3) O(4) nanoparticles were then synthesized by carbodiimide reaction of propargylamine with the carboxyl groups on the surface of the iron oxide nanoparticles. By further reaction with N(3) -bacitracin in a Cu(I) -catalyzed azide-alkyne cycloaddition, the magnetic Fe(3) O(4) nanoparticles were modified with the peptide bacitracin. The functionalized magnetic nanoparticles were characterized by powder X-ray diffraction, X-ray photoelectron spectroscopy, TEM, zeta-potential analysis, FTIR spectroscopy and vibrating-sample magnetometry. Cell cytotoxicity tests indicate that bacitracin-conjugated Fe(3) O(4) nanoparticles show very low cytotoxicity to human fibroblast cells, even at relatively high concentrations. In view of the antibacterial activity of bacitracin, the biofunctionalized Fe(3) O(4) nanoparticles exhibit an antibacterial effect against both Gram-positive and Gram-negative organisms, which is even higher than that of bacitracin itself. The enhanced antibacterial activity of the magnetic nanocomposites allows the dosage and the side effects of the antibiotic to be reduced. Due to the antibacterial effect and magnetism, the bacitracin-functionalized magnetic nanoparticles have potential application in magnetic-targeting biomedical applications.
[Show abstract][Hide abstract] ABSTRACT: Industrial and urban activities yield large amounts of contaminated groundwater, which present a major health issue worldwide. Infectious diseases are the most common health risk associated with drinking-water and wastewater remediation is a major concern of our modern society. The field of wastewater treatment is being revolutionized by new nano-scale water disinfection devices which outperform most currently available technologies. In particular, iron oxide magnetic nanoparticles (MNPs) have been widely used in environmental applications due to their unique physical-chemical properties. In this work, poly(ethylene) glycol (PEG)-coated MNPs have been functionalized with (RW)3, an antimicrobial peptide, to yield a novel magnetic-responsive support with antimicrobial activity against Escherichia coli K-12 DSM498 and Bacillus subtilis 168. The magnetic-responsive antimicrobial device showed to be able to successfully disinfect the surrounding solution. Using a rapid high-throughput screening platform, the minimal inhibitory concentration (MIC) was determined to be 500 μM for both strains with a visible bactericidal effect.
Water Research 08/2014; 66C:160-168. DOI:10.1016/j.watres.2014.08.024 · 5.53 Impact Factor
"Moreover, nanoparticles with smaller particle sizes have been shown to possess antimicrobial properties . The antimicrobial activity of iron oxide nanoparticles has largely been studied against different organisms [1, 5–7] and has been shown to depend on three factors: size, stability, and concentration in the growth medium. The size of microbial cells is in the micrometer range while outer cellular membranes have pores in the nanometer range. "
[Show abstract][Hide abstract] ABSTRACT: Nystatin is a tetraene diene polyene antibiotic showing a broad spectrum of antifungal activity. In the present study, we prepared
a nystatin nanocomposite (Nyst-CS-MNP) by loading nystatin (Nyst) on chitosan (CS) coated magnetic nanoparticles (MNPs).
The magnetic nanocomposites were characterized by X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy
(FT-IR), thermogravimetry analysis (TGA), vibrating sample magnetometer (VSM), and scanning electron microscopy (SEM).
The XRD results showed that the MNPs and nanocomposite are pure magnetite. The FTIR analysis confirmed the binding of CS
on the surface of theMNPs and also the loading of Nyst in the nanocomposite. The Nyst drug loading was estimated using UV-Vis
instrumentation and showing a 14.9% loading in the nanocomposite. The TEM size image of the MNPs, CS-MNP, and Nyst-CSMNPwas
13, 11, and 8 nm, respectively.The release profile of theNyst drug fromthe nanocomposite followed a pseudo-second-order
kineticmodel.The antimicrobial activity of the as-synthesizedNyst andNyst-CS-MNP nanocomposite was evaluated using an agar
diffusion method and showed enhanced antifungal activity against Candida albicans. In this manner, this study introduces a novel
nanocomposite that can decrease fungus activity on-demand for numerous medical applications.
BioMed Research International 05/2014; 2014(651831,):13 pages. DOI:10.1155/2014/651831 · 3.17 Impact Factor
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.