Molecular detection of tick-borne bacterial agents in Brazilian and exotic captive carnivores

Division of Medical Microbiology, Department of Pathology, Johns Hopkins School of Medicine, 21205 Baltimore, MD, USA.
Ticks and Tick-borne Diseases (Impact Factor: 2.72). 06/2012; 3(4):247-53. DOI: 10.1016/j.ttbdis.2012.04.002
Source: PubMed


The present study aims to detect and characterize by molecular techniques, the presence of tick-borne pathogens in wild captive carnivore blood samples from Brazil. Blood was collected from 76 Brazilian felids, 23 exotic felids, 3 European wolves (Canis lupus), and 97 Brazilian canids maintained in captivity in zoos located in São Paulo and Mato Grosso states, Brazil. DNA of each sample was used in PCR reactions for Ehrlichia, Anaplasma, and Rickettsia identification. The blood from 10/100 (10%) of canids (1 European wolf, 3 bush dogs, and 6 crab-eating foxes) and from 21/99 (21%) felids (4 pumas, 6 little spotted cats, 4 ocelots, 3 jaguarundis, 1 tiger, and 3 lions) contained fragments of 16S rRNA gene of Ehrlichia spp. Fragments of Anaplasma spp. groESL and 16S rRNA genes were detected in the blood of 1/100 (1%) canids (1 bush dog) and in 4/99 (3%) felids (4 little spotted cats), respectively. Rickettsia species infections were not identified. The present work showed that new strains of Ehrlichia and Anaplasma spp. circulate among wild carnivores in Brazil.

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Available from: John Stephen Dumler, Jul 14, 2015
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    • "Although the presence of E. canis infection is likely to occur in both Indian communities (due to the presence of the vector R. sanguineus), it is possible that other ehrlichial agents, associated with native tick species, also occur in the region and therefore could be causing serological cross-reactions with E. canis antigens. In fact, recent studies have reported a variety of ehrlichial agents different from E. canis infecting ticks and animals in different regions of Brazil, indicating that the diversity of Ehrlichia spp. in Brazil is much greater than previously thought (Machado et al. 2007, Widmer et al. 2011, André et al. 2012, Cruz et al. 2012, Machado et al. 2012, Almeida et al. 2013, Aguiar et al. 2014). "
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