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CARACTERIZACIÓN MOLECULAR EN TÉ NURITE Satureja macrostema (Benth) Briq.MEDIANTE MARCADORES RAPDs

Foresta Veracruzana 01/2010; 12(1):27-32.

ABSTRACT Nurite tea is one of the most important medicinal plants for Purepechas. However, in previous works, nurite
tea the taxonomic keys do not correspond with characteristics of the specimens found in the Meseta
Purepecha. Based on the above was necessary to perform studies that help us sort out the relationships in
eight plants known as tea nurit (Satureja macrostema (Benth) Briq.) in Michoacan state, were evaluated using
the technique known as Random Amplified Polymorphic DNA (RAPDs). RAPD fragments generated by
primers OPA01 and OPA10 were analyzed by assigning values of 1 and 0 to the presence or absence of the
respective band originating a binary matrix which was evaluated with the statistic program type AMOVA in this
way constructing a of nearness monophyletic dendrogram. Nurit tea plants have only amplified fragments
among them ± 950 bands that are present in all plants of this species. Nurit tea phenological classified as
white tea turned out to be the same as you nurit classified as black. The rest of the plants are different nurit
are you need a new classification.

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    ABSTRACT: The last decade has witnessed successful applications of plant tissue culture techniques in several crops. During that same period, studies in plant molecular genetics have also grown exponentially. Molecular markers (isozymes, RFLPs, and PCR-based markers such as RAPDs) are now used to study many of the current limitations of tissue culture. They have been used to investigate mechanisms that underlie somaclonal variation in the nuclear, mitochondrial, and chloroplast genomes. One recurrent problem with several tissue culture systems has been the difficulty of determining the origin of regenerants. Molecular markers represent powerful tools to determine precisely the origin of plants derived from microspore or anther culture, protoplast fusion, and other tissue culture studies where this information is important. With improvements in tissue culture techniques, populations of doubled haploid lines have been produced in several major crop species. Doubled haploid populations have proven useful in the production of molecular maps and in tagging important agronomic traits. This review describes the use of molecular markers to address fundamental and practical questions of plant tissue culture, and discusses the potential of improvements in molecular techniques and new molecular markers such as SCAR and STS along with high-resolution mapping strategies.
    In Vitro Cellular & Developmental Biology - Plant 12/1993; 30(1):32-39. · 1.14 Impact Factor

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Jul 1, 2012