Identification of the Anti-oxidants in Flos Chrysanthemi by HPLC-DAD-ESI/MS(n) and HPLC Coupled with a Post-column Derivatisation System.
ABSTRACT INTRODUCTION: Flos Chrysanthemi (Jiju) is a traditional Chinese medicine (TCM) that is known to have anti-oxidant activity; in this study, on-line HPLC-DAD-ESI/MS(n) and HPLC-DAD-DPPH methods have been developed for rapidly screening and identifying free-radical scavengers in Jiju extract. OBJECTIVE: To develop an efficient method for the simultaneous identification and detection of the anti-oxidant components in Flos Chrysanthemi (Jiju). METHODOLOGY: A concentrated methanol extract of Flos Chrysanthemi from Jiaxiang County (Jiju) was first separated into phases soluble in water, petroleum ether and n-butanol. The off-line 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging method was then used to evaluate the anti-oxidant activity of each phase in vitro. The results showed that the n-butanol extract had the highest anti-oxidant activity, and its anti-oxidant compounds were analysed by HPLC-DAD-ESI/MS(n) and HPLC coupled with a post-column derivatisation (PCD) system supplied with DPPH, aluminium chloride or sodium acetate solutions. RESULTS: A total of 17 compounds were separated and identified, three of which were identified in Jiju for the first time, and seven active compounds serve as the chemical basis of the anti-oxidant efficacy of Jiju. CONCLUSION: The methods described here allow rapid separation and convenient identification of the multiple constituents in Jiju, and may be applied to other complex natural matrices. Copyright © 2012 John Wiley & Sons, Ltd.
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ABSTRACT: The in vitro antibacterial activities of a total of 46 extracts from dietary spices and medicinal herbs were investigated by agar-well diffusion method against five foodborne bacteria (Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, and Salmonella anatum). Their total phenolic contents were also evaluated. Many herb and spice extracts contained high levels of phenolics and exhibited antibacterial activity against foodborne pathogens. Gram-positive bacteria were generally more sensitive to the tested extracts than Gram-negative ones. S. aureus was the most sensitive, while E. coli was the most resistant. There were highly positive relationships (R(2)=0.73-0.93) between antibacterial activities and phenolic content of the tested extracts against each bacterium. This suggested that the antibacterial activity of the tested extracts was closely associated with their phenolic constituents.International Journal of Food Microbiology 07/2007; 117(1):112-9. · 3.43 Impact Factor
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ABSTRACT: Eleven tabular and nine ligulate flowers from 15 species of Compositae plants were investigated for their triterpene alcohol constituents. This led to the isolation and identification of 11 triterpene alcohols as follows: heliaol, taraxasterol, psi-taraxasterol, alpha-amyrin, beta-amyrin, lupeol, taraxerol, cycloartenol, 24-methyl-enecycloartanol, tirucalla-7,24-dienol and dammaradienol. The tabular flowers of Calendula officinalis, Carthamus tinctorius, Cosmos bipinnatus, Chrysanthemum morifolium, Helianthus annuus and Matricaria matricarioides showed a characteristic feature by containing helianol as the most predominant component (29-86%) in the triterpene alcohol fractions. The triterpene alcohols from Compositae flowers were evaluated with respect to their anti-inflammatory activity against 12-O-tetradecanoylphorbol-13-acetate-induced inflammation (1 microgram per ear) in mice. All of these showed marked inhibitory activity, and their 50% inhibitory dose was 0.1-0.8 mg per ear.Phytochemistry 01/1997; 43(6):1255-60. · 3.05 Impact Factor
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ABSTRACT: Ion trap/time-of-flight hybrid mass spectrometers are powerful tools for the detailed structural analysis of modified peptides. We have analyzed Met-Lys-bradykinin modified with deoxycholate at the amino-terminus or the epsilon-amino group as model peptides. These two modified peptides produced fragment ions with the same nominal but different exact masses in tandem mass spectrometry with low-energy collision-induced dissociation. Accurate high-resolution analysis coupled with MS(3) allowed us to distinguish the deoxycholate modification sites in the modified peptides.Analytical and Bioanalytical Chemistry 11/2006; 386(3):682-8. · 3.66 Impact Factor