Article

Age-related decrease of the LAMP-2 gene expression in human leukocytes.

Shandong Provincial Key Laboratory of Cardiac Disease Diagnosis and Treatment, Jining Medical College Affiliated Hospital, Jining Medical College, Jining, Shandong 272029, China.
Clinical biochemistry (impact factor: 2.02). 06/2012; 45(15):1229-32. DOI:10.1016/j.clinbiochem.2012.06.016 pp.1229-32
Source: PubMed

ABSTRACT Autophagy is a highly conserved degradation pathway in cells, which has been involved in many physiological processes and implicated in human age-related diseases. However, autophagy activities have not been systemically investigated with human tissues and cells.
Lysosomal associated membrane protein-2 (LAMP-2) protein is critical for autophagy and chaperone-mediated autophagy. We examined LAMP-2 gene expression and protein levels in the peripheral leukocytes from healthy subjects over 40years old.
Compared to those in group of 40-44years, the LAMP-2 transcript and protein levels in groups of 65-69 (P<0.01) and over 70years (P<0.001) were significantly decreased. No significant difference in LAMP-2 transcript and protein levels were observed between male and female groups.
Our data revealed that there was a progressive and age-related decrease of the LAMP-2 gene expression in the peripheral leukocytes of healthy subjects, indicating a trend of decreasing autophagy activities with aging.

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Keywords

40years old
 
age-related decrease
 
autophagy activities
 
conserved degradation pathway
 
decreasing autophagy activities
 
female groups
 
healthy subjects
 
human age-related diseases
 
human tissues
 
LAMP-2
 
LAMP-2 gene expression
 
LAMP-2 transcript
 
membrane protein-2
 
peripheral leukocytes
 
physiological processes
 
protein levels
 

Jian Huang