Article

Background exposure to toxic metals in women adversely influences pregnancy during in vitro fertilization (IVF)

Department of Environmental Health Sciences, University at Albany, State University of New York, Rensselaer, NY, USA.
Reproductive Toxicology (Impact Factor: 2.77). 06/2012; 34(3):471-81. DOI: 10.1016/j.reprotox.2012.06.002
Source: PubMed

ABSTRACT Low-level environmental exposure to Hg, Pb and Cd may interfere with pregnancy during in vitro fertilization (IVF). The aim of this study was to generate hypotheses concerning associations between background exposures and pregnancy. In modified Poisson regression models including 24 women and adjusted for urine Cd and creatinine, blood Pb, age, race and smoking, 1 μg/L increases in blood Hg are associated with decreases of 35% (P=0.03) and 33% (P=0.01) in clinical and biochemical pregnancies, respectively. In alternate Poisson models including 26 women and adjusted for blood Pb, blood Hg, age, race and smoking, 1 μg/L increases in blood Cd are associated with decreases of 94% (P=0.01) and 82% (P=0.04) in clinical and biochemical pregnancies, respectively. No effects are detected in 15 men, although inverse associations are suggested for urine cadmium and pregnancy. These data suggest that low-level, background exposures to Hg and Cd may interfere with pregnancy following IVF.

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Available from: Michael S Bloom, Aug 29, 2015
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    • "A future study incorporating the results of a sperm chromatin structure assay (SCSA) will help us to interpret these associations in a more definitive fashion. Previously we reported a negative association between blood Hg and embryo quality (Bloom et al., 2011) and no associations for pregnancy (Bloom et al., 2012). However, our current results suggest positive associations for SP Hg and embryo quality, implantation, pregnancy, and live birth. "
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    ABSTRACT: We measured toxic metals in seminal plasma collected from 30 men using vitro fertilization (IVF), to evaluate associations with semen quality and IVF outcomes. A doubling in Hg-adjusted Pb concentration was associated with 47% lower total motile sperm. Positive associations were suggested for Hg with pregnancy and live birth, adjusted for Cd or Pb. A negative association was suggested for Hg-adjusted Cd with pregnancy. These data add to evidence indicating that toxic metals impact IVF.
    Environmental Research 08/2014; 133:334-337. DOI:10.1016/j.envres.2014.06.014 · 3.95 Impact Factor
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    • "A future study incorporating the results of a sperm chromatin structure assay (SCSA) will help us to interpret these associations in a more definitive fashion. Previously we reported a negative association between blood Hg and embryo quality (Bloom et al., 2011) and no associations for pregnancy (Bloom et al., 2012). However, our current results suggest positive associations for SP Hg and embryo quality, implantation, pregnancy, and live birth. "
    American Society for Reproductive Medicine, Boston, MA; 10/2013
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    ABSTRACT: PURPOSE: We previously reported associations between trace concentrations of Hg, Cd and Pb in blood and urine and reproductive outcomes for women undergoing in-vitro fertilization (IVF). Here we assess measurements in single follicular fluid (FF) specimens from 46 women as a presumably more relevant marker of dose for reproductive toxicity. METHODS: FF specimens were analyzed for Hg, Cd and Pb using sector field-inductively coupled plasma-mass spectrometry (SF-ICP-MS). Variability sources were assessed by nested ANOVA. Multivariable regression was used to evaluate associations for square root transformed metals with IVF outcomes, adjusting for confounders. RESULTS: An inverse association is detected for FF Pb and fertilization (relative risk (RR) = 0.68, P = 0.026), although positive for Cd (RR = 9.05, P = 0.025). While no other statistically significant associations are detected, odds ratios (OR) are increased for embryo cleavage with Hg (OR = 3.83, P = 0.264) and Cd (OR = 3.18, P = 0.644), and for embryo fragmentation with Cd (OR = 4.08, P = 0.586) and Pb (OR = 2.22, P = 0.220). Positive estimates are observed for Cd with biochemical (RR = 19.02, P = 0.286) and clinical pregnancies (RR = 38.80, P = 0.212), yet with very low precision. CONCLUSIONS: We have identified associations between trace amounts of Pb and Cd in FF from a single follicle, and oocyte fertilization. Yet, the likelihood of biological variation in trace element concentrations within and between follicles, coupled with levels that are near the limits of detection suggest that future work should examine multiple follicles using a 'one follicle-one oocyte/embryo' approach. A larger study is merited to assess more definitively the role that these environmental factors could play with respect to egg quality in IVF programs.
    Journal of Assisted Reproduction and Genetics 11/2012; 29(12). DOI:10.1007/s10815-012-9882-z · 1.77 Impact Factor
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