Multifunctional Materials through Modular Protein Engineering

Department of Bioengineering, Stanford University, CA 94305, USA.
Advanced Materials (Impact Factor: 17.49). 08/2012; 24(29):3923-40. DOI: 10.1002/adma.201200051
Source: PubMed


The diversity of potential applications for protein-engineered materials has undergone profound recent expansion through a rapid increase in the library of domains that have been utilized in these materials. Historically, protein-engineered biomaterials have been generated from a handful of peptides that were selected and exploited for their naturally evolved functionalities. In recent years, the scope of the field has drastically expanded to include peptide domains that were designed through computational modeling, identified through high-throughput screening, or repurposed from wild type domains to perform functions distinct from their primary native applications. The strategy of exploiting a diverse library of peptide domains to design modular block copolymers enables the synthesis of multifunctional protein-engineered materials with a range of customizable properties and activities. As the diversity of peptide domains utilized in modular protein engineering continues to expand, a tremendous and ever-growing combinatorial expanse of material functionalities will result.

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    • "Moreover, proteins usually serve as cross-linkers via covalent (Michael addition [15], [16], enzyme reaction [17] or site selective conjugation [18], [19]) or non-covalent interactions (specific protein-peptide [20], protein-protein [21], [22] or protein-polysaccharide interactions [23]) in protein-based hydrogels, which require them to have multiple binding sites to their ligands. Some specific amino acid side chain groups such as the lysine’s ε-amine and cysteine’s sulphydryl endow favourable targets for cross-linking reactions [24], [25]. "
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    PLoS ONE 09/2014; 9(9):e107949. DOI:10.1371/journal.pone.0107949 · 3.23 Impact Factor
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    Chemical Society Reviews 11/2012; 42(3). DOI:10.1039/c2cs35358j · 33.38 Impact Factor
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    ABSTRACT: Cells respond to their environment in complex and sometimes poorly understood ways. Protein, peptide and synthetic peptidomimetic ligands may all be used to stimulate cells via receptor signaling, using interactions that are often highly specific. Polymer substrates that present these ligands provide a promising way to control cell development, both for applications in biotechnology and for fundamental studies of cell biology. Here we review a large range of techniques that have been employed to create and characterize ligand-functionalized substrates, with a particular focus on techniques that allow specific and consistent stimulation.
    Progress in Polymer Science 01/2013; 39(7). DOI:10.1016/j.progpolymsci.2013.11.006 · 26.93 Impact Factor
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