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Development and application of a fluoride-detection-based fluorescence assay for γ-butyrobetaine hydroxylase.

Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
ChemBioChem (Impact Factor: 3.74). 06/2012; 13(11):1559-1563. DOI:10.1002/cbic.201200256
Source: PubMed

ABSTRACT Fluoride assays for oxygenases: The 2-oxoglutarate-dependent oxygenase BBOX catalyses the final step in carnitine biosynthesis and is a medicinal chemistry target. We report that BBOX can hydroxylate fluorinated substrates analogues with subsequent release of a fluoride ion, thereby enabling an efficient fluorescence-based assay.

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May 1, 2013