Article

Cleavage and protection of locked nucleic acid-modified DNA by restriction endonucleases.

School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Brisbane, Queensland 4072, Australia.
Bioorganic & medicinal chemistry letters (impact factor: 2.65). 06/2012; 22(14):4836-8. DOI:10.1016/j.bmcl.2012.05.113 pp.4836-8
Source: PubMed

ABSTRACT Locked nucleic acid (LNA) is one of the most prominent nucleic acid analogues reported so far. We herein for the first time report cleavage by restriction endonuclease of LNA-modified DNA oligonucleotides. The experiments revealed that RsaI is an efficient enzyme capable of recognizing and cleaving LNA-modified DNA oligonucleotides. Furthermore, introduction of LNA nucleotides protects against cleavage by the restriction endonucleases PvuII, PstI, SacI, KpnI and EcoRI.

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Keywords

cleavage
 
cleaving LNA-modified DNA oligonucleotides
 
efficient enzyme capable
 
first time report cleavage
 
LNA
 
LNA nucleotides
 
LNA-modified DNA oligonucleotides
 
prominent nucleic acid analogues
 
PstI
 
restriction endonucleases PvuII