Antifungal activity of coronarin D against Candida albicans. Oral Surg Oral Med Oral Pathol Oral Radiol

Instructor, Department of Oral Diagnosis, Faculty of Dentistry, Naresuan University, Phitsanulok, Thailand.
Oral surgery, oral medicine, oral pathology and oral radiology 07/2012; 114(1):61-6. DOI: 10.1016/j.oooo.2012.01.010
Source: PubMed


The objective of this study was to investigate the antifungal activity of coronarin D on Candida albicans and its activity was compared with clotrimazole and nystatin.
Coronarin D was extracted by liquid chromatography and used in antifungal testing. The inhibitory effect of coronarin D on C. albicans was determined by cultures and an applied broth dilution test. The rate of fungicidal activity was evaluated by time-kill curves. Morphologic alterations of fungal cells were investigated using scanning electron microscopy.
Coronarin D was effective against C. albicans; the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) were 2 and 4 mg/mL, respectively. The C. albicans killing activity of coronarin D was higher than clotrimazole and nystatin at 2×MFC and 4×MFC, respectively. Morphologic alterations of fungal cells consistent with cell membrane damage were observed in the coronarin D-treated cells.
Coronarin D showed promising antifungal activity against C. albicans in vitro.

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    • "Various biological activities of coronarin D were observed, for example, cytotoxic activity against cancer cell [10, 11] and inhibiting both constitutive and inducible nuclear factor-kappa B pathway, a key mediator of inflammation, apoptosis, invasion, and osteoclastogenesis [12]. Recently, antifungal activity of coronarin D against Candida albicans has just been reported [13]. This encouraged coronarin D to be of interest for studying its activity against different microorganisms. "
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    ABSTRACT: Coronarin D is a labdane-type diterpene from the rhizomes of Hedychium coronarium. In the view of our ongoing effort to explore its novel biological activity, antimicrobial activity study of coronarin D was performed. The results showed that coronarin D was active against tested Gram-positive bacteria, inactive for tested Gram-negative bacteria, and weakly active against tested fungi. The antibacterial effect of the combination of coronarin D with nine classical antibiotics against four Gram-positive bacteria was also evaluated. The fractional inhibitory concentration indices (FICI) of coronarin D-antibiotics combinations, calculated from the checkerboard assay, were used as synergism indicator. Out of 36 combinations, 47% showed total synergism, 33% had partial synergistic interaction, 17% showed no effect, and 3% showed antagonism. By combination with coronarin D at concentration of 0.25 minimal inhibitory concentration (MIC), the activities of antibiotics were boosted to 4- to 128-fold. These finding suggested an attractive approach to combat the infectious diseases by using coronarin D-antibiotic drug combination.
    BioMed Research International 05/2014; 2014(14):581985. DOI:10.1155/2014/581985 · 3.17 Impact Factor
  • Edible Medicinal and Non Medicinal Plants, 01/2014: pages 847-856; , ISBN: 978-94-017-8747-5