RP-HPLC Method for Simultaneous Estimation of Atorvastatin Calcium and Ramipril from Capsule Dosage Form

Inventi Rapid: Pharm Ana & Qual Assur 05/2010; 1(1):1-3.


INTRODUCTION Atorvastatin [R-(R*, R*)]-2-(4-fluorophenyl)-β,∂ dihydroxy-5-(1-methylethyl)-3-phenyl-4-[(phenyl amino) carbonyl]-1H-pyrrole-1-heptanoic acid calcium salt for trihydrate] is Anti-Lipidemic agent act by inhibiting HMG CoA reductase enzyme. [1] Ramipril [2S-[1[R*(R*)], 2α, 3αβ, 6αβ]]-1-[2[[1(Ethoxycarbonyl)-3-phenylpropyl] amino]-1-oxon propyl] octahydro cyclopenta [b] pyrrole-2-carboxylic acid.] is ACE inhibitor. [2-3] The combination of both drugs is used in treatment of Hypertension. Several HPLC [4-6] , HPLC [7] , Liquid Chromatography– electro Spray Ionization Tandem Mass Spectrometry [8] methods have been reported for atorvastatin alone or in combination with other drugs. [9-11] Only one UV spectroscopy i.e. derivative spectroscopy method has been reported for simultaneous estimation of atorvastatin (ATR) and ramipril (RAM). [12] The present method (Internal Standard RP-HPLC method) quantitates both drugs simultaneously without exipients interference in analysis from capsule dosage form [13] MATERIALS AND METHODS: Instrumentation: The separation of ATR and RAM was carried out on an isocratic JASCO RP-HPLC system using C18 column (150x4.6mm internal diameter, particle size 5µm).The pump used in this HPLC system was PU 2080 pump (Dual piston with gear driven pump). The 20 µl sample solutions were injected to chromatographic system using Rheodyne

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