Article

Effects of the presence or absence of a protein corona on silica nanoparticle uptake and impact on cells.

Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Dublin 4, Ireland.
ACS Nano (impact factor: 10.77). 06/2012; 6(7):5845-57. DOI:10.1021/nn300223w pp.5845-57
Source: PubMed

ABSTRACT Nanoparticles enter cells through active processes, thanks to their capability of interacting with the cellular machinery. The protein layer (corona) that forms on their surface once nanoparticles are in contact with biological fluids, such as the cell serum, mediates the interactions with cells in situ. As a consequence of this, here we show that the same nanomaterial can lead to very different biological outcomes, when exposed to cells in the presence or absence of a preformed corona. In particular, silica nanoparticles exposed to cells in the absence of serum have a stronger adhesion to the cell membrane and higher internalization efficiency, in comparison to what is observed in medium containing serum, when a preformed corona is present on their surface. The different exposure conditions not only affect the uptake levels but also result in differences in the intracellular nanoparticle location and impact on cells. Interestingly, we also show that after only one hour of exposure, a corona of very different nature forms on the nanoparticles exposed to cells in the absence of serum. Evidence suggests that these different outcomes can all be connected to the different adhesion and surface properties in the two conditions.

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Keywords

active processes
 
biological fluids
 
cell membrane
 
cell serum
 
cellular machinery
 
different adhesion
 
different biological outcomes
 
different exposure conditions
 
different nature forms
 
different outcomes
 
higher internalization efficiency
 
intracellular nanoparticle location
 
Nanoparticles
 
one hour
 
preformed corona
 
silica nanoparticles
 
stronger adhesion
 
surface properties
 
two conditions
 
uptake levels