Y chromosome microsatellite isolation from BAC clones in the greater white‐toothed shrew (Crocidura russula)

Département d’Ecologie et d’Evolution, Université de Lausanne, Bâtiment de Biologie, CH-1015 Lausanne, Switzerland
Molecular Ecology Notes (Impact Factor: 2.38). 11/2005; 6(1):276 - 279. DOI: 10.1111/j.1471-8286.2005.01180.x


We constructed a microsatellite library from four Crocidura russula Y chromosome-specific bacterial artificial chromosome (BAC) clones. Only one of eight microsatellites was male-specific, despite genome walking to obtain more flanking sequence and testing of 93 primer combinations. Potential reasons for this low success are discussed. The male-specific locus, CRY3, was genotyped in 90 males, including C. russula from across the species range and two related species. The large difference in CRY3 allele size between eastern and western lineages supports earlier reports of high divergence between them. Despite polymorphism of CRY3 in Morocco, only one allele was found throughout the whole of Europe, consistent with previous studies that suggest recent colonization of Europe from a small number of Moroccan founders.

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Available from: Lori Lawson Handley, Apr 15, 2014
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    • "Reduced levels of diversity in sex chromosomes compared with autosomes are widespread ( Ellegren 2009 ) , particularly on the Y chromosome ( Kayser et al . 2003 ; Lawson Handley et al . 2006 ; Frankham 2012 ) . This is attributable to lower recombination rates and effective population sizes ( Kayser et al . 2003 ; Lawson Handley et al . 2006 ; Frankham 2012 ) . In M . fuliginosus , the low effective male population size is due to a female - biased sex ratio ( 3 : 1 : Norbury et al . 1988"
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    ABSTRACT: There are several aspects of biology in which the contribution of males and females is unequal. In these instances the examination of Y chromosome markers may be used to elucidate male specific attributes. Here, male dispersal patterns and genetic structuring were examined using four Y-microsatellite loci in 186 male western grey kangaroos (Macropus fuliginosus) from throughout the species' trans-continental distribution. In addition, a sample of both M. fuliginosus and the eastern grey kangaroo (Macropus giganteus) from their region of sympatry in eastern Australia, were examined to determine the male contribution to hybridisation. Detected Y chromosome diversity was low, resulting from low effective male population size due to skewed sex ratios and a polygynous mating system. As expected, male dispersal was high across the range. However, the Lake Torrens/ Flinders Ranges region appears to have significantly restricted male movement between eastern and central/western Australia. There was little evidence to suggest other barriers (Nullarbor Plain and Swan River valley) previously identified by nuclear and mtDNA marker studies restrict male movement. Hence, the admixture events previously identified may be associated with high male dispersal. Within the region of sympatry between M. fuliginosus and M. giganteus in eastern Australia, four M. giganteus individuals were found to possess M. fuliginosus Y-haplotypes. These results confirm the occurrence of hybridisation between male M. fuliginosus and female M. giganteus. Additionally, the introgression of M. fuliginosus Y-haplotypes into M. giganteus populations indicates that at least some male hybrids are fertile, despite evidence to the contrary from captive studies. This study has provided insights into the male contribution population history, structure and hybridisation in M. fuliginosus, which were not predicted via comparisons between biparentally and maternally inherited markers. This highlights the importance of direct examination of the Y chromosome to provide novel insights into male mediated processes, especially where the contribution of the sexes may differ.
    Australian Journal of Zoology 01/2013; 61(1). DOI:10.1071/ZO12087 · 0.94 Impact Factor
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    • "Finally, this method obviously relies on characterizing informative sex-specific markers, and relative lack of variability on the Y chromosome may be a limiting factor here (Hellborg & Ellegren 2004; Hammond et al. 2006; Lawson Handley et al. 2006a,b). However, the increasing availability of Y-linked markers (Hellborg & Ellegren 2003), particularly microsatellites (Erler et al. 2004; Lawson Handley & Perrin 2006) will hopefully facilitate its future use in this type of study. "
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    ABSTRACT: Sex-biased dispersal is an almost ubiquitous feature of mammalian life history, but the evolutionary causes behind these patterns still require much clarification. A quarter of a century since the publication of seminal papers describing general patterns of sex-biased dispersal in both mammals and birds, we review the advances in our theoretical understanding of the evolutionary causes of sex-biased dispersal, and those in statistical genetics that enable us to test hypotheses and measure dispersal in natural populations. We use mammalian examples to illustrate patterns and proximate causes of sex-biased dispersal, because by far the most data are available and because they exhibit an enormous diversity in terms of dispersal strategy, mating and social systems. Recent studies using molecular markers have helped to confirm that sex-biased dispersal is widespread among mammals and varies widely in direction and intensity, but there is a great need to bridge the gap between genetic information, observational data and theory. A review of mammalian data indicates that the relationship between direction of sex-bias and mating system is not a simple one. The role of social systems emerges as a key factor in determining intensity and direction of dispersal bias, but there is still need for a theoretical framework that can account for the complex interactions between inbreeding avoidance, kin competition and cooperation to explain the impressive diversity of patterns.
    Molecular Ecology 04/2007; 16(8):1559-78. DOI:10.1111/j.1365-294X.2006.03152.x · 6.49 Impact Factor
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    ABSTRACT: Microsatellites were identified from three fully sequenced Y chromosome-specific bacterial artificial chromosome clones from the tammar wallaby, Macropus eugenii. Ten microsatellites were genotyped in male tammar wallabies. Four loci were polymorphic with between two and six alleles per locus. Eleven different haplotypes were identified from 22 male tammar wallabies. No amplifications were obtained from female samples. Each microsatellite was also shown to amplify reliably in at least one other macropod species. These markers may therefore prove useful as some of the first male-specific genetic markers for marsupials, with potential application to studies of male-biased dispersal and mating systems.
    Molecular Ecology Notes 11/2006; 6(4):1202 - 1204. DOI:10.1111/j.1471-8286.2006.01491.x · 2.38 Impact Factor
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