Rotavirus genotypes in Slovenia: Unexpected detection of G8P and G12P genotypes
ABSTRACT A rotavirus surveillance study was undertaken in Slovenia from December 2005 to March 2006. Stool samples from 114 children hospitalized with acute viral gastroenteritis were collected from two main Slovenian hospitals. These confirmed rotavirus-positive samples were selected for a rotavirus G and P genotype prevalence study. Six untypable strains of genotype G were further analyzed with sequencing of the VP7, VP8*, and NSP4 genes. The findings of the study were that the G1 genotype was the most prevalent, found in 72 samples (63.2%), followed by G9 in 26 samples (22.8%), G4 in 10 samples (8.8%), and G3 in 2 samples (1.7%). All G genotypes were combined with the P genotype specificity. After sequence analysis, one G8 and two G12 genotypes were also characterized. In a VP7-based phylogenetic analysis, the G8P strain (SI-885/06) was more closely related to the Cody I801 bovine strain than to other human strains. Both G12 strains (SI-264/06 and SI-403/06) were shown to belong to the Se585 G12 cluster. In the VP8* phylogenetic tree, all analyzed strains except one, belonged to the P lineage II and shared high identity in amino acid sequence. All characterized strains were clustered into the NSP4 genotype B. The molecular characterization of this G8 strain supports the theory of interspecies transmission of rotaviruses and animal-human genome reassortment. This is the first report on rotavirus G12 detection in Slovenia. J. Med. Virol. 79:626–632, 2007. © 2007 Wiley-Liss, Inc.
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ABSTRACT: To assess diversity of rotavirus strains in Lilongwe, Malawi, we conducted a cross-sectional study of children with acute gastroenteritis, July 2005-June 2007. Serotype G12 was identified in 30 (5%) of 546 rotavirus-positive fecal specimens. The G12 strain possessed multiple electropherotypes and P-types, but their viral protein 7 sequences were closely related, indicating that reassortment has occurred.Emerging Infectious Diseases 02/2009; 15(1):87-90. · 6.79 Impact Factor
Article: Molecular epidemiology of rotaviruses in Bulgaria: annual shift of the predominant genotype.[show abstract] [hide abstract]
ABSTRACT: Rotavirus molecular epidemiology investigations provide important information about the incidence of rotavirus diseases and rotavirus strains in circulation in the prevaccine era. The purpose of this investigation was to study the burden of rotavirus disease, rotavirus strain diversity, and epidemiology specificities of rotavirus infections in Bulgaria. A total of 3,130 stools collected between 2005 and 2008 were tested by immune enzyme tests. G-P genotype identification of rotavirus strains were performed by reverse transcriptase polymerase chain reaction (RT-PCR). Rotavirus etiology was confirmed in 32.4% of the samples tested. Rotaviruses affected predominantly children under 5 years of age (95.5%), with a peak prevalence between the ages of 7 and 36 months. Four of the five globally distributed rotavirus strains (G1P, G2P, G4P, and G9P) constituted 97.7% of all rotavirus strains in circulation. However, annual shifts of predominant rotavirus G-P genotypes were observed from season to season-G4P was predominant in rotavirus season 2004/2005 (56.8%), but was replaced by G9P in 2005/2006 (77.7%), and G2P (41.6%) and G1P (39.5%) in the following two consecutive rotavirus seasons. Year-round circulation of rotaviruses in the country with increased incidence in the winter-spring season and unexpected peaks preceding the rotavirus seasons were observed. Molecular epidemiology data are needed in Bulgaria for health policy makers in order to introduce routine rotavirus vaccination. The monitoring of rotavirus genetic diversity in Bulgaria in the postvaccination period will contribute to a successful rotavirus vaccination program.European Journal of Clinical Microbiology 03/2010; 29(5):555-62. · 2.86 Impact Factor
Article: Identification of lineage III of G12 rotavirus strains in diarrheic children in the Northern Region of Brazil between 2008 and 2010.[show abstract] [hide abstract]
ABSTRACT: This study reports on the surveillance for rotavirus genotypes and the identification of G12 human rotavirus in the Northern Region of Brazil. Rotavirus-positive samples were collected from children <5 years of age with acute diarrhea from January 2008 to October 2010. G2P was the most prevalent genotype, accounting for 45.6% (126/303) of cases. Five rotavirus strains bearing G12P genotype specificity were detected. Phylogenetic analysis of the VP7 gene showed that G12 strains clustered into lineage III. This is the first detection of G12 strains from lineage III in Latin America, broadening the current evidence for the worldwide emergence of this genotype.Archives of Virology 09/2011; 157(1):135-9. · 2.11 Impact Factor