The survival of lactic acid bacteria in rumen fluid

Department of Bacteriology, University of Wisconsin, Madison, WI, USA
Journal of Applied Microbiology (Impact Factor: 2.39). 05/2003; 94(6):1066 - 1071. DOI: 10.1046/j.1365-2672.2003.01942.x

ABSTRACT Aims: To determine whether lactic acid bacteria (LAB) used in inoculants for silage can survive in rumen fluid (RF), and to identify those that survive best.
Methods and Results: Twelve commercial silage inoculants were added at 107 CFU ml−1 to strained RF (SRF) taken from dairy cows, with and without 5 g l−1 glucose and incubated in vitro at 39°C. Changes in pH, LAB numbers and fermentation products were monitored for 72 h. In the inoculated RF with glucose, the pH decreased and numbers of LAB increased. The inoculants varied with regard to their effect on pH change and growth. In the SRF, both with and without glucose, the pH values of the inoculated samples were generally higher than those of the uninoculated controls throughout most of the incubation period. This may suggest a positive effect on the rumen environment.
Conclusions: LAB used in silage inoculants can survive in RF in vitro.
Significance and Impact of the Study: This is the first step in studying the probiotic potential of silage LAB inoculants for dairy cattle. The survival of these LAB in RF may enable them to interact with rumen microorganisms and to affect rumen functionality.

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Available from: Paul J Weimer, Aug 26, 2015
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    • "The effect of LAB on the rumen environment has been studied in a number of experiments. Lactic acid bacteria can survive during in vitro ruminal incubation and potentially affect volatile fatty acids (VFA) composition (Weinberg et al., 2003, 2004). Muck et al. (2007) demonstrated that microbial silage inoculants had an effect on in vitro ruminal gas and VFA production, but effects differed by inoculant. "
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    • "When the silo is opened, the anaerobic environment is changed to an aerobic one and microorganisms which remain dormant in the absence of air multiply, resulting in a deterioration of silages, especially in warm climates (Woolford, 1990; Ashbell et al., 2002). Many workers have found that aerobic deterioration resulted from the activity of aerobic bacteria, yeast and mold utilizing residue WSC and lactic acid producing a rising pH and energy loss and even the possibility of producing harmful by products (Honig et al., 1980; MuDonald et al., 1991; Weinberg et al., 2003). Therefore, less pH change indicates good aerobic stability of silage. "
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    • "Energy crops and crop residues can relatively easily be stored by ensiling. Ensiling is a biochemical process which converts the soluble carbohydrates contained in the plant matter to lactic acid, acetate, propionate, and butyrate which inhibit the growth of detrimental microorganisms by a strong drop in pH to values between 3 and 4 (Weinberg et al. 2003). "
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