Panorama of DNA hairpin folding observed via diffusion-decelerated fluorescence correlation spectroscopy.

Beijing National Laboratory for Molecular Sciences and Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Chemical Communications (Impact Factor: 6.72). 06/2012; 48(59):7413-5. DOI: 10.1039/c2cc31986a
Source: PubMed

ABSTRACT Based on a confocal microscopy platform, we extended the FCS time window by three orders of magnitude to the s timescale by attaching a polystyrene microsphere. We simultaneously monitored the relaxations of multiple intermediates involved in DNA hairpin folding, thus offering a much more detailed view of the kinetics of hairpin folding experimentally.

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