Article

Sensitivity enhancement of fiber-laser-based stimulated Raman scattering microscopy by collinear balanced detection technique.

Division of Advanced Science and Biotechnology, Osaka University, Suita, Osaka 565-0871, Japan.
Optics Express (impact factor: 3.59). 06/2012; 20(13):13958-65. pp.13958-65
Source: PubMed

ABSTRACT We propose the collinear balanced detection (CBD) technique for noise suppression in fiber laser (FL)-based stimulated Raman scattering (SRS) microscopy. This technique reduces the effect of laser intensity noise at a specific frequency by means of pulse splitting and recombination with a time delay difference. We experimentally confirm that CBD can suppress the intensity noise of second harmonic (SH) of Er-FL pulses by 13 dB.The measured noise level including the thermal noise is higher by only ~1.4 dB than the shot noise limit. To demonstrate SRS imaging, we use 4-ps SH pulses and 3-ps Yb-FL pulses, which are synchronized subharmonically with a jitter of 227 fs. The effectiveness of the CBD technique is confirmed through SRS imaging of a cultured HeLa cell.

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Keywords

CBD
 
CBD technique
 
collinear balanced detection
 
cultured HeLa cell
 
Er-FL pulses
 
intensity noise
 
laser intensity noise
 
noise level
 
noise suppression
 
pulse splitting
 
Raman scattering
 
second harmonic
 
shot noise limit
 
SRS
 
SRS imaging
 
synchronized subharmonically
 
thermal noise
 
time delay difference