The Elongate loach (Leptobotia elongata) belongs to family Cobitidae, which is endemic to the middle and upper reaches of the Yangtze River in China. In this study, the complete mitochondrial genome of L. elongata was sequenced. It was determined to be 16,591 bases. The nucleotide sequence data of 12 heavy-strand protein-coding genes of L. elongata and other 12 Cobitidae species were used for phylogenetic analyses. Trees constructed using Bayesian and maximum parsimony showed a similar topology demonstrating that L. elongate was clustered in subfamily Botiinae being sister to the subfamily Cobitinae. The trees also suggested that the genera Cobitis were polyphyletic.
"Bayesian inference (BI), maximum likelihood (ML) and maximum parsimony (MP) frameworks were used to examine the phylogenetic position of M. altaica (Figure 1). The parameters and methods to build the three different phylogenetic trees were followed Li et al. (2012) and Lu et al. (2013). The different tree-building methods (MP, ML and Bayesian methods) yielded the same topology, with strong support for all nodes (Figure 1). "
[Show abstract][Hide abstract] ABSTRACT: Abstract The mountain weasel (Mustela altaica) belongs to family Mustelidae, which is the near threatened species in the IUCN Red List. In this study, the complete mitochondrial genome of M. altaica was sequenced and characterized. The genome is 16,521 bases in length (GenBank accession no. KC815122). The nucleotide sequence data of 12 heavy-strand protein-coding genes of M. altaica and other 20 Mustelidae species were used for phylogenetic analyses. Trees constructed by using Bayesian inference, maximum parsimony and maximum likelihood demonstrated that M. altaica was close to Mustela nivalis and they were sister to Mustela putorius and Mustela sibirica.
Mitochondrial DNA 06/2013; 25(4). DOI:10.3109/19401736.2013.800491 · 1.21 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Abstract The Leptobotia microphthalma belongs to family Cobitidae, which is endemic to the upper reaches of the Yangtze River in China. In this studying, the complete mitochondrial genome sequence of L. microphthalma has been obtained with PCR, which contains 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and a non-coding control region with the total length of 16,512 bp. The gene arrangement and composition are similar to that of other vertebrates. Most of the genes are encoded on heavy strand, except for eight tRNA and ND6 genes. The complete mitochondrial genome sequence of L. microphthalma would contribute to better understand population genetics and protect its genetic diversity.
Mitochondrial DNA 07/2013; 25(4). DOI:10.3109/19401736.2013.800495 · 1.21 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Abstract In this study, the complete mitogenome sequence of balitorid fish, giant stone loach Triplophysa siluroides, which collected from the Yellow River, China has been sequenced by the long polymerase chain reaction method. The mitogenome, consisting of 16,574 base pairs (bp), had typical vertebrate mitochondrial gene arrangement, including 13 protein coding genes, 22 transfer RNAs, 2 ribosomal RNA genes and a noncoding control region (CR). CR of 918 bp length is located between tRNA(Pro) and tRNA(Phe). The overall base composition of Triplophysa siluroides is 28.8% for A, 28.7% for T, 25.0% for C, and 17.5% for G, with a slight AT bias of 57.5%. The complete mitochondrial genome may provide rather informative data for reconstructing and addressing new molecular perspectives for balitorid phylogenies.
Mitochondrial DNA 06/2014; DOI:10.3109/19401736.2014.926523 · 1.21 Impact Factor
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