A New Marine Ciliate, Metaurostylopsis antarctica nov spec. (Ciliophora, Urostylida) from the Antarctic Ocean

Acta protozoologica (Impact Factor: 1.18). 12/2011; 50:289–300. DOI: 10.4467/16890027AP.11.026.0063

ABSTRACT In this study, a new marine urostylid ciliate, Metaurostylopsis antarctica nov. spec. collected from the Antarctic Ocean was investigated using morphological, morphometrical, and molecular methods. Metaurostylopsis antarctica nov. spec. is characterized as follows: slender to ellipsoid form in body shape; two types of cortical granules, ellipsoid large one (type I, yellow-green, 1.5 × 1 μm) in rows along dorsal kineties and cirri, circular small one (type II, colourless, 0.3 μm in diameter) scattered throughout whole body; 19–24 adoral membranelles, 4 frontal cirri, 2–5 frontoterminal cirri, 1 buccal and 2 transverse cirri; 3–5 midventral pairs, 10–15 cirri of midventral row; 1 right and 2 left marginal rows; 3 dorsal kineties; about 43 macronuclear nodules. This new species mainly differs from the congeners by the number of marginal rows (1 vs. 3 or more on right side; 2 vs. 3 or more on left side). In addition, proter’s oral primordium developed on the right side of the oral cavity (vs. in center of oral cavity), and the rightmost anlage splits into two parts, namely, the frontoterminal cirri and a transverse cirrus (vs. only frontoterminal cirri). Inter-specific dissimilarities of the SSU rRNA gene between the congeners range from 3.3 to 4.4%.

  • [Show abstract] [Hide abstract]
    ABSTRACT: Two new species of Bakuella, B. (B.) incheonensisn. sp. and B. (Pseudobakuella) litoralisn. sp., were discovered in brackish water near AamdoShore Park, Incheon, South Korea. We conducted a morphological study based on live observations and protargol-impregnated specimens, and a molecular analysis using nuclear SSU rRNA gene sequences.Bakuella(B.) incheonensisis diagnosed by:body size 70-105 × 20-40μm in vivo, 21-25 adoralmembranelles, 3 or4 frontoterminal cirri, midventral complex composed of 7-10 midventralpairs with 1 or2 rows and terminatingat about 62% of body length, 20-28 left and 25-32 right marginal cirri, 58-87 macronuclear nodules, and yellowish cortical granules.Bakuella(Pseudobakuella) litoralisis diagnosed by: body size 90-125 × 30-40 μm in vivo, 25-33 adoralmembranelles, 3-5 buccal cirri, midventral complex composed 10-15midventral pairs with 1 or2 rows and terminated at 70% of body length, 1 or 2 pretransverse cirri, 3-6 transverse cirri, 26-39 left and 29-47 right marginal cirri, 49-84 macronuclear nodules, andtwo types of cortical granules. Molecular phylogeny using SSU rRNA gene sequences shows a non-monophyletic relationship amongBakuellaspecies and emphasizes the need for further morphogeneticstudies of this genus and otherrelated hypotrichs. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
    Journal of Eukaryotic Microbiology 05/2015; DOI:10.1111/jeu.12238 · 2.91 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Two hypotrichous ciliates from China were investigated. The common oxytrichid species Tetmemena pustulata (Müller, 1786) Eigner, 1999, isolated from the estuary of the Pearl River in southern China, was investigated with emphasis on its living morphology and infraciliature. Tetmemena pustulata is characterized as follows: body elliptical to obovoid in shape; 75–115 × 40–60 μm in vivo; two macronuclear nodules and two micronuclei; one contractile vacuole left of midline and somewhat ahead of midbody positioned; three frontal, four frontoventral, one buccal, three postoral ventral, two pretransverse ventral and five transverse cirri; cirrus III/2 ahead of level of cirrus IV/3; cirrus IV/2 arranged more anteriorly than cirrus V/4; transverse cirri not forming two distinct groups; three prolonged and widely separated caudal cirri; six dorsal kineties in Oxytricha-pattern with dorsal kineties 3 and 4 bipolar. The marine urostylid species Metaurostylopsis salina Li et al., 2005, isolated from an aquarium in Qingdao, northern China, was investigated with emphasis on its morphogenesis which is characterized by the de novo formation of the oral primordium in the proter and the development of the marginal rows from two anlagen that form within each parental structure separately in both dividers.
    European Journal of Protistology 05/2013; 49(2):272–282. DOI:10.1016/j.ejop.2012.07.003 · 2.34 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: This paper investigates the morphology, infraciliature, and small subunit (SSU) rRNA gene sequences of two hypotrichous ciliates, Neourostylopsis orientalis n. sp., and Protogastrostyla sterkii (Wallengren, 1900) n. comb., collected from coastal waters in southern China. Neourostylopsis orientalis n. sp. is mainly diagnosed by the arrangement of brownish cortical granules, the number of adoral membranelles, frontal and transverse cirri, and the characteristics of its midventral cirral pairs. The SSU rRNA phylogenies strongly support the establishment of the new genus Neourostylopsis, which is mainly characterized by the following features: frontal and transverse cirri clearly differentiated, buccal cirri present, two frontoterminal cirri, midventral complex composed of midventral pairs only and not exceeding the halfway point of the cell, more than one row of marginal cirri on each side which derive from individual anlagen within each parental row, caudal cirri lacking. Thus, two new combinations have to be made: Neourostylopsis songi (Lei et al., 2005) n. comb., and Neourostylopsis flavicana (Wang et al., 2011) n. comb. Additionally, improved diagnoses for both Metaurostylopsis and Apourostylopsis are supplied in this study. Protogastrostyla sterkii (Wallengren, 1900) n. comb. differs from the similar congener, P. pulchra, mainly in the body shape, ratio of buccal field to body length in vivo, and molecular data. Based on the present studies, we conclude that the estuarine population of Protogastrostyla pulchra collected by Gong et al. (2007) is a population of P. sterkii.
    International Journal of Systematic and Evolutionary Microbiology 01/2013; DOI:10.1099/ijs.0.049403-0 · 2.80 Impact Factor