Article

Exogenous NADP increases the level of histone H2AX phosphorylation in mouse heart cells after ionizing radiation

Cell and Tissue Biology 04/2012; 5(4):383-387. DOI:10.1134/S1990519X11040055

ABSTRACT Phosphorylation of replacement histone H2AX occurs in megabase chromatin domains around DNA double-strand breaks (DSBs), and
this modification called γ-H2AX can be used as an effective marker for DSBs repair and DNA damage response. Using Western
blotting and immunohistochemistry techniques we have studied here the influence of exogenous nicotinamide adenine dinucleotide
phosphate (NADP), which can potentially increase the level of intracellular NAD+, on the level of γ-H2AX formation in mouse heart cells after ionizing radiation (IR). We have found that injection of NADP
in different doses immediately after IR causes an increased level of γ-H2AX in mouse heart cells 20 min after IR at the dose
of 3 Gy compared to control mice after IR exposure. It indicates that there could be a relationship between intracellular
NAD+ content and DNA damage response in vivo.

KeywordsIonizing radiation–Histone H2AX phosphorylation–NADP–DNA damage response

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Keywords

control mice
 
different doses
 
DNA damage response
 
DNA double-strand breaks
 
effective marker
 
intracellular NAD+
 
ionizing radiation
 
IR
 
IR causes
 
IR exposure
 
KeywordsIonizing radiation–Histone H2AX phosphorylation–NADP–DNA damage response
 
megabase chromatin domains
 
mouse heart cells
 
mouse heart cells 20 min
 
replacement histone H2AX
 
γ-H2AX
 
γ-H2AX formation