Role of endogenous porphyrins in the effects of low-intensity laser radiation of the red region on free radical processes in the blood of rats under experimental endotoxic shock

Abstract

The role of endogenous porphyrins in the effects of laser radiation of the red region (632.8 nm) on free radical processes
in the blood of rats under endotoxic shock induced by the administration of lipopolysaccharide B (25 mg/kg) has been studied.
The measurements of the functional activity of polymorphonuclear leukocytes (the method of luminol-dependent chemiluminescence),
the superoxide dismutase activity of blood plasma (using nitro blue tetrazolium), and the degree of lipid oxidation of erythrocyte
membranes (the method of fluorescence of cis-parinaric acid) have been carried out. It has been found that low-intensity laser radiation strongly affects all processes
examined irrespective of the administration of lipopolysaccha-ride B. The effect of radiation was most pronounced in animals
injected with the polysaccharide, the changes being dependent on the concentration of endogenous porphyrins in samples.

Keywordsendotoxic shock–low-intensity laser radiation–endogenous porphyrins–reactive oxygen species–leukocytes–superoxide dismutase activity–free radical lipid oxidation