Article

In vitro evaluation of the antioxidant activities in the differentially processed seeds from underutilized legume, Bauhinia vahlii Wight & Arn

Food science and biotechnology (Impact Factor: 0.66). 04/2010; 19(2):503-509. DOI: 10.1007/s10068-010-0070-6

ABSTRACT Antioxidant potential and total phenolics content of 70% acetone extracts of the raw and processed seeds of Bauhinia vahlii were evaluated. The extract of raw seeds contained higher levels of total phenolics (30.8 g/100 g) and tannins (19.6 g/100
g) compared to dry heated and soaking followed by autoclaving seed extracts. Extracts were screened for antioxidant and free
radical scavenging activities using various chemical and in vitro model systems. In all the models, except DPPH radical scavenging activity, the extract from raw seeds manifested the strongest
antioxidant activity than that from processed seeds. In β-carotene/linoleic acid emulsion system and superoxide scavenging
activity, the raw seed extract registered more activity when compared to the standards (butylated hydroxyanisole and α-tocopherol).
Whereas, the extract from dry heated seed exhibited higher DPPH· scavenging activity (IC50 70.77 μg/mL) than the raw seeds (IC50 74.4 μg/mL). This study has to some extent validated the antioxidant potential of the seeds of B. vahlii.

Keywords
Bauhinia vahlii
-polyphenol-antioxidant activity-β-carotene-2,2-diphenylpicryl-1-picryl-hydrazyl (DPPH·)

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    • "Phenolic compounds and antioxidant activities in legume seeds were reported by several earlier communications, although legumes constitute one of the most abundant and least expensive sources of protein in human/animal diet [1]. Among natural antioxidants, phenolics form the largest group. "
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    • "One milliliter of extract (250 μg/ml) was mixed with 2.4 ml of 0.1 M phosphate buffer (pH 7.4), and then 0.6 ml of a 43 mM solution of H 2 O 2 in the same buffer were added (Sowndhararajan et al., 2010). After 40 min, the absorbance of reaction mixture was taken at 230 nm against a blank solution (phosphate buffer without H 2 O 2 ). "
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