In vitro evaluation of the antioxidant activities in the differentially processed seeds from underutilized legume, Bauhinia vahlii Wight & Arn
ABSTRACT Antioxidant potential and total phenolics content of 70% acetone extracts of the raw and processed seeds of Bauhinia vahlii were evaluated. The extract of raw seeds contained higher levels of total phenolics (30.8 g/100 g) and tannins (19.6 g/100
g) compared to dry heated and soaking followed by autoclaving seed extracts. Extracts were screened for antioxidant and free
radical scavenging activities using various chemical and in vitro model systems. In all the models, except DPPH radical scavenging activity, the extract from raw seeds manifested the strongest
antioxidant activity than that from processed seeds. In β-carotene/linoleic acid emulsion system and superoxide scavenging
activity, the raw seed extract registered more activity when compared to the standards (butylated hydroxyanisole and α-tocopherol).
Whereas, the extract from dry heated seed exhibited higher DPPH· scavenging activity (IC50 70.77 μg/mL) than the raw seeds (IC50 74.4 μg/mL). This study has to some extent validated the antioxidant potential of the seeds of B. vahlii.
-polyphenol-antioxidant activity-β-carotene-2,2-diphenylpicryl-1-picryl-hydrazyl (DPPH·)
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ABSTRACT: The methanolic extract of ox-eye bean [Mucuna gigantea (Willd) DC.] contained total free phenolic content of 14.80±1.28 g catechin equivalent/100 g extract dry matter. Encouraging levels of ferric reducing/antioxidant power (FRAP, 1,023 mmol Fe[II]/mg extract), inhibition of β-carotene degradation (59.35%) and radical scavenging activity against DPPH (72.12%) and superoxide (43.11%) were exhibited by the raw samples. Further, it also recorded 82.17% of α-amylase and 91.26% of α-glucosidase enzyme inhibition characteristics. Sprouting+oil-frying caused a apparent increase on the total free phenolic content and also significant improvement on the antioxidant and free radical scavenging capacity of methanolic extract, while soaking+cooking as well as open-pan roasting treatments showed diminishing effects. Moreover, inhibition of α-amylase and α-glucosidase enzyme activities was declined to 22.82 and 45.47%, respectively during sprouting+oilfrying treatment, which are more desirable for the dietary management of type II diabetic patients. Keywordsox-eye bean (Mucuna gigantean)–total free phenolics–antioxidant activity–α-amylase inhibition–α-glucosidase inhibition–indigenous processing methodFood science and biotechnology 20(3):783-791. · 0.70 Impact Factor
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ABSTRACT: The objectives of this study were to determine phenolic content and antioxidant activities of chloroform, acetone, methanol and hot water extracts of Bauhinia vahlii leaves. The hot water extract afforded the highest yield (6.3%) while the lowest yield was obtained from the chloroform extract (2.1%). The methanol extract contains higher levels of total phenolics (48.7 ± 0.7 g GAE/100 g extract), tannins (21.7 ± 0.7 g GAE/100 g extract) and flavonoids (10.3 ± 0.2 RE/100 g extract). The extracts were subjected to assess their antioxidant potential using various in vitro systems such as DPPH(•), ABTS(•+), FRAP, OH(•), β-carotene linoleic acid bleaching system, phosphomolybdenum reduction and Fe(2+) chelation. It is concluded that the methanolic extract of B. vahlii leaves have strong antioxidant potential. Further study is necessary for isolation and characterization of the active antioxidants, which may serve as a potential source of natural antioxidants.Saudi Journal of Biological Sciences 10/2013; 20(4):319-25.
Conference Paper: JERS INSAR DEM generation for Borneo[Show abstract] [Hide abstract]
ABSTRACT: The Sebuku-Sembakung area of north-eastern Kalimantan, Indonesia (Borneo) has been proposed by the WWF as a national park because of its large biodiversity with habitats including various types of tropical forests and swamps. Currently, only low resolution DEM data exists for the area. This has motivated an effort to produce a topographic map of the region using L-band repeat pass interferometry with JERS-1 data. Significant coverage exists for the area and passes were selected on the basis of coverage, time interval and baseline. Regions with sufficient interferometric correlation for DEM production over the 44 day repeat interval are mangrove and tidal swamp forests. Other surface classes including peat swamp, lowland mixed and hill Dipterocarp forest have lower correlation that precludes generation accurate topography from the available JERS-1 data in these areasGeoscience and Remote Sensing Symposium, 2000. Proceedings. IGARSS 2000. IEEE 2000 International; 02/2000