Article

Comparison of Two On-Line Analysis Techniques Used for the Screening of Antioxidants in EGb 761

Key Laboratory of Modern Chinese Medicines, China Pharmaceutical University, 210009 Nanjing, China
Chromatographia (impact factor: 1.2). 04/2012; 71(5):493-497. DOI:10.1365/s10337-010-1470-8 pp.493-497

ABSTRACT In order to perform on-line screening procedures for antioxidants in the Ginkgo biloba extract (EGb761), a comparison was carried out between LC–DAD–DPPH analysis used for the determination of DPPH· bleaching and LC–DAD–CL determination applied for the detection of H2O2 and O2−· scavenging activities. The results demonstrated that 15 antioxidants in EGb 761 could be identified by LC–DAD–CL, while only
10 antioxidants were detected under LC–DAD–DPPH conditions. Furthermore, it was found that both sensitivity and reproducibility
of the LC–DAD–CL method were superior. The results indicated that the combination of the two methods might provide useful
information for the prediction of antioxidants in herbal medicines.

KeywordsColumn liquid chromotography-Chemiluminescence-1,1-Diphenyl-2-picrylhydrazyl-Free radicals-Antioxidants-EGb 761

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Keywords

10 antioxidants
 
15 antioxidants
 
antioxidants
 
DPPH· bleaching
 
Ginkgo biloba
 
KeywordsColumn liquid chromotography-Chemiluminescence-1,1-Diphenyl-2-picrylhydrazyl-Free radicals-Antioxidants-EGb 761
 
LC–DAD–CL method
 
LC–DAD–DPPH conditions
 
O2−· scavenging activities
 
on-line screening procedures
 
reproducibility
 
two methods
 

Xiao-Ping Ding