Article

Overexpression of FAD2 promotes seed germination and hypocotyl elongation in Brassica napus

Plant Cell Tissue and Organ Culture (impact factor: 3.09). 04/2012; 102(2):205-211. DOI:10.1007/s11240-010-9723-7

ABSTRACT The enzyme fatty acid desaturase 2 (FAD2) transforms oleic acid (C18:1) to linoleic acid (C18:2) in plants and as such is
involved in fatty acid synthesis. It is also involved in plant development and self-defense, such as seed germination, leaf
expansion and cold resistance. We have cloned the full coding region of the Brassica napus
FAD2 gene and ectopically expressed it in B. napus expressing low levels of FAD2. Overexpression of FAD2 under the control of the CaMV 35S promoter resulted in an up-regulated FAD2 mRNA level in B. napus as expected. Further analysis revealed that the FAD2 transgenic lines varied greatly in terms of their physiological characteristics, such as enhanced seed germination and increased
hypocotyl length, compared to non-transgenic plants, suggesting that up-regulated FAD2 can promote seed germination and hypocotyl elongation in B. napus. Our results demonstrate the possible roles of FAD2 in plant development and also provide a platform for further analysis of fatty acid synthesis in plants.

Keywords
Brassica napus
-Fatty acid desaturase 2-Hypocotyl length-Overexpression-Seed germination-Transgenic plant

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Keywords

B. napus
 
CaMV 35S promoter
 
cold resistance
 
enhanced seed germination
 
enzyme fatty acid desaturase 2
 
FAD2 transgenic lines varied
 
fatty acid synthesis
 
full coding region
 
hypocotyl elongation
 
hypocotyl length
 
linoleic acid
 
low levels
 
non-transgenic plants
 
physiological characteristics
 
plant development
 
plants
 
possible roles
 
seed germination
 
up-regulated FAD2
 
up-regulated FAD2 mRNA level
 

Maohua Wang