Identification of QTLs for powdery mildew and scald resistance in barley

Shtaya Faculty of Agriculture, An-Najah N. University, P.O. Box 707, Nablus, Palestinian Territory
Euphytica (Impact Factor: 1.39). 11/2006; 151(3):421-429. DOI: 10.1007/s10681-006-9172-x
Source: OAI


A population of 103 recombinant inbred lines (RILs, F9-derived lines) developed from the two-row spring barley cross L94 × ‘Vada’ was evaluated under field conditions for resistance against powdery mildew (Blumeria graminis f.sp. hordei) and scald (Rhynchosporium secalis). Apart from the major resistance gene mlo on chromosome 4 (4H), three QTLs (Rbgq1, Rbgq2 and Rbgq3) for resistance against powdery mildew were detected on chromosomes 2 (2H), 3 (3H), and 7 (5H), respectively. Rbgq1 and Rbgq2 have not been reported before, and did not map to a chromosome region where a major gene for powdery mildew had been reported. Four QTLs (Rrsq1, Rrsq2, Rrsq3 and Rrsq4) for resistance against scald were detected on chromosomes 3 (3H), 4 (4H) and 6 (6H). All four mapped to places where QTLs for scald resistance had been reported before in different populations.

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Available from: Diego Rubiales, Sep 29, 2015
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    • "In the Czech Republic, the mean annual area of winter barley during 2010–2012 was 103 000 ha. Winter barley, whose long growing season includes a period of low temperatures, is infected by more pathogens than spring barley. Foliar diseases are a major constraint to winter barley production, especially net blotch (Lei sov a et al. 2006), leaf scald (Shtaya et al. 2006, Looseley et al. 2012), Ramularia leaf spot (Matu sinsky et al. 2013) and spot blotch (Matu sinsky et al. 2010, Lei sov a-Svobodov a et al. 2012b). However, the dominant disease on susceptible winter barley cvs. is powdery mildew caused by the airborne biotrophic ascomycete Blumeria graminis f. sp. "
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    ABSTRACT: In central Europe, winter barley is infected by a broader spectrum of pathogens than spring barley. However, the dominant disease on susceptible cultivars is powdery mildew. The objective of this contribution was to postulate resistance genes in cultivars registered in the Czech Republic and Slovakia from 1993 to 2010 using a set of 40 isolates of the barley powdery mildew pathogen. Seedlings of 42 European cultivars were tested, and 15 known resistance genes were postulated namely, Mla6, Mla7, Mla12, Mla13, MlaRu4, Mlg, Mlh, MlLa, Mlra, Ml(Ch), Ml(Dr2), Ml(IM9), Ml(Lo), Ml(Ru2) and Ml(St). Two unknown resistances were detected, one in cultivars Gilberta, Mirko and Polana, and another in Ricus. Three cultivars (Campanile, Carrero and Mirko) were heterogeneous for mildew response. The most frequent genes were Mlra and Mlh, which were found in 25 and 14 cultivars, respectively. Genes located at the Mla locus were present in 14 cultivars; 11 of these cultivars carried Mla6. No resistance gene was detected in Hanna. Previously unknown resistances will be further characterized.
    Plant Breeding 12/2013; 132(6). DOI:10.1111/pbr.12108 · 1.60 Impact Factor
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    • "This fact may lead to a minor-gene-for-minor-gene interaction between the QTL of the plant and a gene factor conditioning aggressivity or " partial virulence " on the pathogen. Rrsq1, on chromosome 3H, was mapped at a region where a QTL was detected in the same population for partial resistance to scald under field condition (Shtaya et al., 2006) and in three other mapping populations (Thomas et al., 1995; Spaner et al., 1998; Grønnerød et al., 2002, Hayes et al., 2001). "
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    ABSTRACT: A population of 103 recombinant inbred lines (RILs) of barley, that were developed from the cross L94x’Vada’ was evaluated at seedling stage for resistance against scald (Rhynchosporium secalis) in a sporeproof air-conditioned glasshouse. Two isolates of R. secalis were used: B9 and 13-13. Both isolates were collected in fields at SCRI (Scottish Crop Research Institute) near Dundee, Scotland, UK. Under glasshouse conditions, two quantitative trait loci (QTLs) for partial resistance (PR) to scald were identified. One QTL (Rrsq1) was detected on chromosome 3 (3H) for latency period and lesion length only against B9 isolate, whereas the other QTL (Rrsq2, on chromosome 7H) was identified for lesion length of both isolates. Rrsq1 was mapped at a region where a QTL was detected, using the same population, for partial resistance to scald under field conditions whereas Rrsq2 was mapped at a similar position as the locus Rrs2 for scald resistance. These cases of co-location of QTL with major genes, not very common, may suggest that some QTL are actually alleles of known major resistance genes.
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    • "They cause a reduced rate of epidemic development despite a high, susceptible, infection type (Parlevliet and Kuiper 1977; Shtaya et al. 2006). Even though the effects of QTL alleles may vary with the environment (Chelkowski et al. 2003), they are nonetheless considered today as a more durable source of resistance (Qi et al. 1999, 2000; Shtaya et al. 2006). Identifying novel major resistance loci and quantitative loci that can be combined with known genes is paramount for a sustainable resistance breeding (Williams 2003). "
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    ABSTRACT: Powdery mildew and scald can cause significant yield loss in barley. In order to identify new resistance genes for powdery mildew and scald in barley, two barley doubled haploid (DH) populations were screened for adult plant resistance in the field and glasshouse under natural infection. The mapping populations included 92 DH lines from the cross of TX9425×Franklin and 177 DH lines from the cross of Yerong×Franklin. Two quantitative trait loci (QTL) for resistance to powdery mildew were identified in the TX9425×Franklin population. These QTL were mapped to chromosomes 7H and 5H, respectively. The phenotypic variation explained by the two QTL detected in this population was 22 and 17%, respectively. Three significant QTL were identified from the Yerong×Franklin population for the resistance to powdery mildew; the major one, detected on the short arm of chromosome 1H, explained 66% of phenotypic variation. The major QTL for scald resistance, identified from two different populations which shared a common parent, Franklin, were mapped in the similar position on 3H. However, the Franklin allele provided resistance to one population but susceptibility to the other population. The Yerong allele on 3H showed much better resistance to scald than the Franklin allele, which has not been reported before. Using high-density maps for both populations, some markers which were very close to the resistance genes were identified. Transgression beyond the parents in disease resistances of the DH populations indicates that both small-effect QTLs and genetic background may also have significant contributions towards the resistance. KeywordsBarley–QTL–Powdery mildew–Scald
    Molecular Breeding 04/2010; 27(4):479-490. DOI:10.1007/s11032-010-9445-x · 2.25 Impact Factor
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