Article

Effects of using a metal layer in total internal reflection fluorescence microscopy

Applied Physics A (impact factor: 1.63). 04/2012; 89(2):333-335. DOI:10.1007/s00339-007-4119-1 pp.333-335

ABSTRACT This proceeding examines the characteristics of imaging through a metal-coated glass cover slip using total internal reflection
fluorescence microscopy (TIRFM). Through back and front focal plane imaging of sub-diffraction-limited fluorescent beads,
the experimental characteristics of the emission are compared with theoretical simulations. Furthermore, at the angle at which
surface plasmon resonance occurs, we show that the anisotropic emission of the fluorescent beads collected through the metal
layer results in a irregular point spread function that has a donut-like structure with multiple concentric rings.

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Keywords

anisotropic emission
 
fluorescent beads
 
focal plane imaging
 
imaging
 
irregular point spread function
 
metal-coated glass
 
multiple concentric rings
 
sub-diffraction-limited fluorescent beads
 
surface plasmon resonance