Effects of using a metal layer in total internal reflection fluorescence microscopy
ABSTRACT This proceeding examines the characteristics of imaging through a metal-coated glass cover slip using total internal reflection
fluorescence microscopy (TIRFM). Through back and front focal plane imaging of sub-diffraction-limited fluorescent beads,
the experimental characteristics of the emission are compared with theoretical simulations. Furthermore, at the angle at which
surface plasmon resonance occurs, we show that the anisotropic emission of the fluorescent beads collected through the metal
layer results in a irregular point spread function that has a donut-like structure with multiple concentric rings.