Mineral Trioxide Aggregate-based Endodontic Sealer Stimulates Hydroxyapatite Nucleation in Human Osteoblast-like Cell Culture

Department of Restorative Dentistry, Dental School of São Paulo State University, Araraquara, São Paulo, Brazil.
Journal of endodontics (Impact Factor: 3.38). 07/2012; 38(7):971-6. DOI: 10.1016/j.joen.2012.02.018
Source: PubMed


The main purpose of this study was to evaluate the biocompatibility and bioactivity of a new mineral trioxide aggregate (MTA)-based endodontic sealer, MTA Fillapex (MTA-F; Angelus, Londrina, Brazil), in human cell culture.
Human osteoblast-like cells (Saos-2) were exposed for 1, 2, 3, and 7 days to MTA-F, Epiphany SE (EP-SE; SybronEndo, Orange, CA), and zinc oxide-eugenol sealer (ZOE). Unexposed cultures were the control group (CT). The viability of the cells was assessed by MTT assay and the morphology by scanning electron microscopy (SEM). The bioactivity of MTA-F was evaluated by alkaline phosphatase activity (ALP) and the detection of calcium deposits in the culture with alizarin red stain (ARS). Energy-dispersive X-ray spectroscopy (EDS) was used to chemically characterize the hydroxyapatite crystallites (HAP). Saos-2 cells were cultured for 21 days for ARS and SEM/EDS. ARS results were expressed as the number of stained nodules per area. Statistical analysis was performed with analysis of variance and Bonferroni tests (P < .01).
MTA-F exposure for 1, 2, and 3 days resulted in increased cytotoxicity. In contrast, viability increased after 7 days of exposure to MTA-F. Exposure to EP-SE and ZOE was cytotoxic at all time points. At day 7, ALP activity increase was significant in the MTA-F group. MTA-F presented the highest percentage of ARS-stained nodules (MTA-F > CT > EP-SE > ZOE). SEM/EDS analysis showed hydroxyapatite crystals only in the MTA-F and CT groups. In the MTA-F group, crystallite morphology and chemical composition were different from CT.
After setting, the cytotoxicity of MTA-F decreases and the sealer presents suitable bioactivity to stimulate HAP crystal nucleation.


Available from: Loise Pedrosa Salles, Aug 10, 2015
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    • "Several properties of MTA FillApex such as biocompatibility [7] [8], bioactivity [9], cytotoxicity [10], solubility [11] [12] [13], "
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    ABSTRACT: The solubility, pH, electrical conductivity, and radiopacity of AH Plus and MTA FillApex were evaluated. In addition, the surfaces morphologies of the sealers were analyzed by using scanning electron microscopy. For pH test, the samples were immersed in distilled water at different periods of time. The same solution was used for electrical conductivity measurement. The solubility and radiopacity were evaluated according to ANSI/ADA. Statistical analyses were carried out at 5% level of significance. MTA FillApex presented higher mean value for solubility and electrical conductivity. No significant difference was observed in the mean values for pH reading. AH Plus presented higher radiopacity mean values. MTA FillApex presented an external surface with porosities and a wide range of sizes. In conclusion, the materials fulfill the ANSI/ADA requirements when considering the radiopacity and solubility. AH Plus revealed a compact and homogeneous surface with more regular aspects and equal particle sizes.
    05/2014; 2014(12):589732. DOI:10.1155/2014/589732
    • "However in these cases, we have used MTA based sealer (MTA Fillapex) to seal the apical foramen or apex of root canal implant-dentin interface at the apex to minimize failure due to leakage. They stimulate the healing process of perapical tissues.[7] "
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    ABSTRACT: Endodontic implants were introduced back in 1960. Endodontic implants enjoyed few successes and many failures. Various reasons for failures include improper case selection, improper use of materials and sealers and poor preparation for implants. Proper case selection had given remarkable long-term success. Two different cases are being presented here, which have been treated successfully with endodontic implants and mineral trioxide aggregate Fillapex (Andreaus, Brazil), an MTA based sealer. We suggest that carefully selected cases can give a higher success rate and this method should be considered as one of the treatment modalities.
    04/2014; 5(1):70-3. DOI:10.4103/0975-5950.140183
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    • "Recent data reported that MTA Fillapex presents acceptable physical properties, antibacterial properties and intracanal resistance to dislodgement similar to AH Plus2,23. The sealer presents increased cytotoxicity during the setting reaction which decreases over time26. After setting, the sealer is capable of retaining alkaline pH levels due to calcium release, and presents bioactivity stimulating hydroxyapatite crystal nucleation in human osteoblast-like cell cultures26 and mineralization in connective tissue14. "
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    Journal of applied oral science: revista FOB 03/2013; 21(2). DOI:10.1590/1678-7757201302254 · 0.92 Impact Factor
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