Mineral trioxide aggregate-based endodontic sealer stimulates hydroxyapatite nucleation in human osteoblast-like cell culture.
ABSTRACT The main purpose of this study was to evaluate the biocompatibility and bioactivity of a new mineral trioxide aggregate (MTA)-based endodontic sealer, MTA Fillapex (MTA-F; Angelus, Londrina, Brazil), in human cell culture.
Human osteoblast-like cells (Saos-2) were exposed for 1, 2, 3, and 7 days to MTA-F, Epiphany SE (EP-SE; SybronEndo, Orange, CA), and zinc oxide-eugenol sealer (ZOE). Unexposed cultures were the control group (CT). The viability of the cells was assessed by MTT assay and the morphology by scanning electron microscopy (SEM). The bioactivity of MTA-F was evaluated by alkaline phosphatase activity (ALP) and the detection of calcium deposits in the culture with alizarin red stain (ARS). Energy-dispersive X-ray spectroscopy (EDS) was used to chemically characterize the hydroxyapatite crystallites (HAP). Saos-2 cells were cultured for 21 days for ARS and SEM/EDS. ARS results were expressed as the number of stained nodules per area. Statistical analysis was performed with analysis of variance and Bonferroni tests (P < .01).
MTA-F exposure for 1, 2, and 3 days resulted in increased cytotoxicity. In contrast, viability increased after 7 days of exposure to MTA-F. Exposure to EP-SE and ZOE was cytotoxic at all time points. At day 7, ALP activity increase was significant in the MTA-F group. MTA-F presented the highest percentage of ARS-stained nodules (MTA-F > CT > EP-SE > ZOE). SEM/EDS analysis showed hydroxyapatite crystals only in the MTA-F and CT groups. In the MTA-F group, crystallite morphology and chemical composition were different from CT.
After setting, the cytotoxicity of MTA-F decreases and the sealer presents suitable bioactivity to stimulate HAP crystal nucleation.
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ABSTRACT: AIM: To assess the current usage and opinions of mineral trioxide aggregate (MTA) amongst European postgraduates (PG) in paediatric dentistry. STUDY DESIGN: An on-line structured questionnaire. METHODS: The programme directors of all EAPD-accredited programmes in paediatric dentistry were contacted by e-mail and invited to disseminate the web-link for a structured on-line questionnaire to all PG students on their programmes. The survey sought details of usage of MTA in both the primary and permanent dentitions and also, previous and future educational requirements for cement use. RESULTS: Responses were received from 29/44 (F: 27; M: 2) PG students. MTA was used in the primary dentition by 6/29 and 17/29 PG for pulp capping and pulpotomy procedures, respectively. In non-vital permanent teeth, MTA was used for apical barrier formation by 26/29 PG students, 15/29 for pulpotomy procedures and 10/29 to repair root perforations. Barriers to the use of MTA related to material cost and other materials being deemed more appropriate. Overall, 27/29 demonstrated an interest in further educational opportunities in material use. CONCLUSION: Mineral trioxide aggregate appears to be in regular use amongst PG dentists in paediatric dentistry throughout Europe.European Archives of Paediatric Dentistry. Official Journal of the European Academy of Paediatric Dentistry. 04/2013;
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ABSTRACT: The aim of this study was to evaluate and compare several physicochemical properties including working and setting times, flow, solubility, and water absorption of a recent calcium silicate-based sealer (MTA Fillapex; Angelus, Londrina, Brazil) and an epoxy resin-based sealer (AH Plus; Dentsply, Konstanz, Germany). The materials were handled following the manufacturer's instructions. The working time and flow were tested according to ISO 6876:2001 and the setting time according to American Society for Testing and Materials C266. For solubility and water absorption tests, the materials were placed into polyvinyl chloride molds (8 × 1.6 mm). The samples (n = 10 for each material and test) were placed in a cylindrical polystyrene-sealed container with 20 mL deionized water at 37°C. At 1, 7, 14, and 28 days, the samples were removed from the solutions and blotted dry for solubility and water absorption tests. The data were analyzed using 1-way analysis of variance with the Tukey test (P < .05). MTA Fillapex showed the lowest values of flow, working and setting times, solubility, and water absorption (P < .05). The solubility and water absorption increased significantly over time for both materials in a 1- to 28-day period (P < .05). MTA Fillapex showed suitable physical properties to be used as an endodontic sealer.Journal of endodontics 07/2013; 39(7):915-8. · 2.95 Impact Factor