[show abstract][hide abstract] ABSTRACT: The effects of salts on cell proliferation in suspension cultures of Avicennia alba and on callus induction from the leaf-tissue of A. marina were investigated using a small-scale liquid culture method. The effects of the seawater components, NaCl, KCl, CaCl2, MgCl2, and MgSO4 were examined separately. In both Avicennia species, the cell growth was increased in the presence of a low concentration, 10 mM, of MgCl2. Even in the presence of 100 mM NaCl, growth was stimulated in A. marina and there was no inhibition of growth in A. alba. CaCl2 was the most inhibitory and completely inhibited growth at 100 mM in both species. Similarities and differences in the effects of sea salts among Avicennia species and Sonneratia alba and Bruguiera sexangula of different mangrove families are discussed. This is the first report on establishing cell suspension culture of the mangrove plant A. alba belonging to the Avicenniaceae.
[show abstract][hide abstract] ABSTRACT: An efficient plant regeneration protocol using axillary shoots of the salt accumulator halophyte, Sesuvium portulacastrum (L.) L. was established and in vitro responses of six Sesuvium clones were studied. The shoot and root induction responses to cytokinins and auxins in clone MH (Maharashtra) were concentration specific. Significantly the highest number of shoots, average shoot elongation and percent shoot regeneration per explant were observed on MS medium supplemented with 40 μM 2-isopentenyl adenine (2iP) followed by 20 μM benzyladenine (BA). Higher cytokinin (60 μM), however, inhibited shoot induction and shoot length. The lower concentrations (5 or 10 μM) of α-napthaleneacetic acid (NAA) proved more effective for root induction, number of roots and average root length. Well-developed plantlets were successfully hardened and established in the field with more than 85 % survival rate. In vitro response of six Sesuvium clones cultured on MS + 20 μM BA revealed higher multiplication rate in clone MH and KA (Karnataka, India) compared to other clones. The results offer the prospect of selecting clones of this species with characteristics desirable for utilization and/or restoration in specific ecological zones.
Physiology and Molecular Biology of Plants 04/2010; 16(2):187-93.
[show abstract][hide abstract] ABSTRACT: Objective: To develop a standard micropropagation protocol for an important mangrove Excoecaria agallocha. Methods: Collection of explants, surface sterilizatio vnu, lpnheernaoblliec cexaurrdieadti oonu ta nind MmMedSi umme dwiausm s tsaunpdpalredmizeendt.e Sdh wooitth i nBdAuPc,t ioKnin, eshtiono, t Zmeautlitnip, l2icipa,t ioNnA Aan, dI AroAo tainngd wIBeAre. Hreamrdoveanli nwga sw raess uclaterrdi eidn 4o ugt/ La fatcetri vraotoetd wcehlalr ceosatal.b Tlihseh emda. xRimeusuml tssh:o Toht ein dbuesctt iopnh ernesopliocn seex usdhoawtioend in MMS medium and better shoot induction was performed in the concentration of BAP (3.9 mol) and NAA (1.34 mol). Rooting induction was performed high range at 5.02 mol of IAA.
Asian Pacific Journal of Tropical Medicine 02/2012; 2(2):S1096–S11018. · 0.50 Impact Factor
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