Inheritance mode of male sterility in bunching onion (Allium fistulosum L.) accessions
ABSTRACT Cytoplasmic male sterility (CMS) is an indispensable trait for F1 hybrid seed production in bunching onion (Allium fistulosum L.). Expansion of the cytoplasmic diversity of F1 hybrid cultivars by introduction of various CMS resources has great potential to eliminate vulnerability to cytoplasm type-specific
diseases. This study aimed to evaluate appearance frequency of male sterile plants in several bunching onion accessions and
to identify CMS resources. In eight (‘Nogiwa Aigara’, ‘Bansei Hanegi’, ‘Amarume’, ‘Kimnung’, ‘Zhangqiu’, ‘INT/CHN/1990/GOTOU’,
‘Natsunegi’ and ‘Guangzhou’) of 135 accessions collected from Japan, China, Mongolia, Korea and Taiwan, male sterile plants
appeared with varied frequencies from 1.7% (‘Nogiwa Aigara’ and ‘Bansei Hanegi’) to 24.5% (‘Zhangqiu’). The inheritance mode
of Zhangqiu- and Guangzhou-derived male sterility was confirmed to be CMS by sib-crossings and interbreed crossings. Microscopic
examination of microsporogenesis in the CMS plants revealed that microspore protoplasm rapidly degenerated without mitotic
division after the release of microspores from tetrads. The CMS germplasm described here would be useful for the development
of “A” lines to be used in F1 hybrid seed production of bunching onion. Male fertility in ‘Nogiwa Aigara’, ‘Bansei Hanegi’, ‘Kimnung’, ‘INT/CHN/1990/GOTOU’
and ‘Natsunegi’ was verified to be controlled by a single fertility restoration locus.
-Bunching onion-CMS-Cytoplasm-Male sterility
- SourceAvailable from: Krystyna Winiarczyk[Show abstract] [Hide abstract]
ABSTRACT: Commercial cultivars of garlic (Allium sativum) do not produce flowers and seed; hence, information on microgametogenesis and genetic knowledge of this important crop is unavailable. Recently, physiological studies enabled flowering and fertility restoration in garlic bolting genotypes by environmental manipulations, thus broadening of the genetic variation and facilitating genetic studies. The present report provides first detailed description of the development of male gametophytes in 11 garlic genotypes varying in their fertility traits. Morphological and anatomical studies revealed completely fertile genotypes, as well as variation in anther and pollen development and disruption of the male organs and gametes at different developmental stages. Three types of plant sterility were observed, including complete sterility, male sterility and environmentally induced male sterility. The ITS1 and ITS2 regions of rRNA of the studied genotypes proved to be strongly conservative and thus did not correspond with the phenotypic expression of fertility or sterility in garlic. On the other hand, two-dimensional protein separation maps revealed significant differences between fertile and sterile genotypes, as well as between developmental stages of microsporogenesis. Further research is needed to investigate the internal mechanisms and environmental component of garlic sterility, as well as the possible molecular markers of these traits.Planta 09/2012; · 3.38 Impact Factor
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ABSTRACT: The subgenus Allium section Allium includes economically important species such as onion (Allium cepa L.), leek (A. ampeloprasum var. porrum L.) and chives (A. schoenoprasum L.). These species have a biennial habit and many cultivars are open pollinated; and high levels of heterozygosity must be maintained. The Alliums’ umbel consists of a few, to hundreds of perfect flowers of per umbel. These flowers are too small to synthetic pollination studies due to the large-scale emasculation is not practical so the development of hybrid varieties is economically feasible using systems of cytoplasmic-genic male sterility (CMS). Male sterile plants, which haven’t got any fertile pollen, incapable of self-pollination so any seed produced must result from cross-pollination. Alliums hybrid production requires the development of male-sterile, maintainer and pollinator lines with good specific combination ability. This review outlines the using male sterility feature in hybrid breeding program for Alliums especially for onions.TABAD Research Journal of Agricultural Sciences ISSN: 1308-3945. 11/2014; 7(2):37-40.