Activité antimicrobienne des huiles essentielles de Pistacia atlantica Desf. de l’Algérie
ABSTRACT L’activité antimicrobienne des huiles essentielles de la résine de Pistacia atlantica Desf., récoltées dans trois stations de l’Ouest et le Sud-Ouest de l’Algérie, a été testée in vitro sur des isolats cliniques
de sept souches bactériennes (Escherichia coli, Enterobacter cloacae, K. pneumoniae, Pseudomonas aeruginosa, Xanthomonas maltophila, Enterococcus feacalis et Staphylococcus aureus) et de trois souches fongiques (Candida albicans, Candida albicans ATCC20027 et Candida albicans ATCC20032). La détermination du pouvoir antibactérien et antifongique a été réalisée par la méthode de la diffusion sur gélose.
Tous les extraits ont montré une forte activité antimicrobienne vis-à-vis des souches fongiques et bactériennes à des concentrations
voisines de 105 µg/ml. Les huiles essentielles ont révélé un grand pouvoir antibactérien vis-à-vis de Staphylococcus aureus et Enterococcus feacalis avec des concentrations minimales inhibitrices (CMI) inférieurs à 10 µg/ml. Candida albicans a présenté une forte résistance aux huiles essentielles testées.
Antimicrobial activity of essential oils of Pistacia atlantica Desf. resin, collected in three localities from the western and south-western of Algeria was tested in vitro on clinical
isolates of seven species of bacteria (Escherichia coli, Enterobacter cloacae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Xanthomonas maltophila, Enterococcus
feacalis, and Staphylococcus aureus) and three species of fungi (Candida albicans, Candida albicans ATCC20027 and Candida albicans ATCC20032). Diffusion method was used for antibacterial and antifungal activities testing. All of the extracts showed strong
antimicrobial activity against both fungi and bacterial strains at the concentration of 105 µg/ml. The essential oils exhibited strong antibacterial activity against Staphylococcus aureus and Enterococcus feacalis with MIC lower than 10 µg/ml. Candida albicans showed resistance to all volatile oils tested.
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ABSTRACT: Essential oils extracted by hydrodistillation from five different varieties of Ocimum basilicum L. plants (Anise, Bush, Cinnamon, Dark Opal and a commercial sample of dried basil) were examined for antimicrobial activity against a wide range of foodborne Gram-positive and -negative bacteria, yeasts and moulds by an agar well diffusion method. All five essential oils of basil showed antimicrobial activity against most of the organisms tested with the exception of Flavimonas oryzihabitans and Pseudomonas species. The inhibitory effect of Anise oil, in comparison with mixtures of the predominant components of pure linalool and methyl chavicol, against the acid-tolerant organisms, Lactobacillus curvatus and Saccharomyces cerevisiae, was examined in broth by an indirect impedance method. Synergistic effects between Anise oil, low pH (pH 4.2) and salt (5% NaCl) were determined. The antimicrobial effect of Anise oil was also assessed in a tomato juice medium by direct viable count, showing that the growth of Lact. curvatus and S. cerevisiae was completely inhibited by 0.1% and 1% Anise oil, respectively. The results of the current study indicate the need for further investigations to understand the antimicrobial effects of basil oils in the presence of other food ingredients and preservation parameters.Letters in Applied Microbiology 04/1998; 26(3):209-14. · 1.63 Impact Factor
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ABSTRACT: The essential oils of leaves (from male and female plants), unripe fruits and leaf-buds of different samples of Pistacia atlantica collected from Greek East Aegean islands (Kalimnos and Lesvos) were analysed by GC–MS and GC. Qualitative and quantitative differences among the samples and different organs of the plant were observed. The oils were rich in monoterpenes. The main components in the leaf oil from the female plants were myrcene (17.8%, 24.8%) and terpinen-4-ol (11.6%, 6.0%) in the Kalimnos and Lesvos samples, respectively, while in the leaf oil from the male plants terpinen-4-ol (17.3% Kalimnos) and p-mentha-1(7),8-diene (41.1%, Lesvos) were the dominant constituents. The major components in unripe fruits were terpinen-4-ol (25.7%, 8.9%), myrcene (20.2%, 34.5%) and sabinene (14.9%, 19.5%). In the leaf-buds oils sabinene (52.1%) and α-pinene (11.6%) were the main constituents in the Kalimnos sample, while in the Lesvos sample the major constituent was p-mentha-1(7),8-diene (42.4%). Copyright © 2007 John Wiley & Sons, Ltd.Flavour and Fragrance Journal 05/2007; 22(5):358 - 362. · 1.82 Impact Factor
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ABSTRACT: One of the best known bioactive triterpenoids is oleanolic acid, a widespread 3-hydroxy-17-carboxy oleanane-type compound. In order to determine whether further oxidation of carbon 3 affects anti-inflammatory activity in mice, different tests were carried out on oleanolic acid and its 3-oxo-analogue oleanonic acid, which was obtained from Pistacia terebinthus galls. The last one showed activity on the ear oedema induced by 12-deoxyphorbol-13-phenylacetate (DPP), the dermatitis induced by multiple applications of 12-O-tetradecanoyl-13-acetate (TPA) and the paw oedemas induced by bradykinin and phospholipase A2. The production of leukotriene B4 from rat peritoneal leukocytes was reduced by oleanonic acid with an IC50 of 17 microM. Negligible differences were observed in the response of both triterpenes to DPP, bradykinin, and phospholipase A2, while oleanonic acid was more active on the dermatitis by TPA and on the in vitro leukotriene formation. In conclusion, the presence of a ketone at C-3 implies an increase in the inhibitory effects on models related to 5-lipoxygenase activity and on associated in vivo inflammatory processes.European Journal of Pharmacology 10/2001; 428(1):137-43. · 2.59 Impact Factor