Clinical significance of Smac expression on non-small cell lung cancers
ABSTRACT ObjectiveThe aim of our study was to investigate the clinical significance of Smac (second mitochondria-derived activator of caspase)
expression on non-small cell lung cancer (NSCLC).
MethodsThe expression of Smac was evaluated on RNA and protein level in tumor tissues. The expression of Smac mRNA was examined by
RT-PCR in 59 samples of tumor tissues and matched normal lung tissues. The expression of Smac protein was examined by IHC
in 213 cancer tissues.
ResultsThe positive rate of Smac mRNA was found in 59.3% of cancer tissues, but only in 30.5% of matched normal tissues (P < 0.05). The positive rate of Smac protein was 76.5%. The expression of Smac in stage II disease was significantly higher
than that in stage I disease (P = 0.001). The survival of patients with Smac overexpression was significantly shorter than those who were negative.
ConclusionSmac might be involved in the progression of NSCLC, the biologic significance of Smac in primary lung cancer needs further
Key wordsSmac–non-small cell lung cancer (NSCLC)–RT-PCR–IHC
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ABSTRACT: Objective The aim of the study was to investigate the expression of Smac protein in human hepatocarcinoma and their relationship with cell apoptosis and proliferation. Methods The expressions of Smac and the proliferating cell nuclear antigen (PCNA) in 41 cancer tissues, 41 adjacent cirrhosis tissues and 9 normal control tissues in hemangioma were assessed by two-step immunohistochemical method and apoptosis was detected by TUNEL method. Results Smac protein was expressed in 14 (34.14%) of the 41 cases of hepatocarcinoma, in 23 (56.10%) of the 41 cases of the adjacent cirrhosis tissues, and in 7 (77.8%) of the normal tissues in hemangioma. Smac protein positive expression rate in hepatocarcinoma was significantly lower than that in the adjacent cirrhosis tissues and the normal control tissues, χ2 were 3.989 and 4.115, respectively, and P were 0.046 and 0.042, respectively. Smac protein expression in cancer was significantly correlated with the ratio of apoptotic index to proliferative index, t′ = 2.260, P < 0.05, but was not with the clinicopathological indicators such as the age and the histological grade, P > 0.05. Conclusion The relatively lower level of the expression of Smac may in a certain extent break the dynamic balance between apoptosis and proliferation of hepatocarcinoma cells, and then plays an important role in the pathogenesis of hepatocarcinoma.The Chinese-German Journal of Clinical Oncology 04/2012; 11(4). DOI:10.1007/s10330-011-0935-z
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ABSTRACT: Brain metastases in patients with lung cancer are a devastating problem with profound impact on survival. Prophylactic cranial irradiation has been discussed as an option to reduce the risk of brain metastases. This report provides an extensive review of the current evidence from non-randomized and randomized trials regarding the use of prophylactic cranial irradiation in lung cancer.Zhongguo fei ai za zhi = Chinese journal of lung cancer 09/2012; 15(9):553-7. DOI:10.3779/j.issn.1009-3419.2012.09.08