Pelger–Huët Anomaly in Australian Shepherds: 87 Cases (1991–1997)
ABSTRACT Blood smears from 892 Australian shepherds were evaluated for the presence of Pelger–Hue¨t (P-H) anomaly over a 6-year period.
During the study, 87 dogs were diagnosed with P-H anomaly (9.8% incidence) following microscopic examination of Wright–Leishman–stained
blood smears. Granulocytes from dogs with P-H anomaly had nuclear hyposegmentation resembling bands and metamyelocytes; however,
the chromatin pattern was more aggregated than that observed in mature segmenters. The granulocyte morphology of dogs with
P-H anomaly was similar to that described for humans and rabbits with the heterozygous form of P-H anomaly. Where gender was
known, 9.4% of males and 8.8% of females had P-H anomaly, indicating autosomal transmittance of the trait. None of the dogs
with P-H anomaly had a predominance of granulocytes with round to oval nuclei (myelocytes) and an extremely coarse chromatin
pattern, suggestive of the homozygous form of the anomaly. Because this phenotype was not observed in Australian shepherds,
the homozygous form of P-H anomaly may be a lethal trait in utero. Six dogs with P-H anomaly had parents with a normal leucocyte
phenotype. In addition, the incidence of P-H anomaly in F1 puppies from matings of individuals with normal and P-H phenotypes
was less than expected. These observations strongly suggest that P-H anomaly is transmitted as an autosomal dominant trait
with incomplete or decreased penetrance in Australian shepherds. Transmittance of P-H anomaly in this breed of dogs may be
governed by more than one allele or expression of the anomaly may modified by genes at a different locus or loci.
- SourceAvailable from: Veronika Šimerdová[Show abstract] [Hide abstract]
ABSTRACT: Pelger-Huët anomaly is an inherited disorder of maturation of myeloid cells, in particular granulocytes and monocytes. Nucleus hyposegmentation, respectively hypolobulation of these cells is the hallmark of Pelger- Huët anomaly. This anomaly is often misinterpreted as inflammatory disease or preleukemic syndrome, which can lead to unnecessary diagnostics and treatment. This case report describes Pelger-Huët anomaly in a two-month-old female Australian Shepherd and is focused on clinical importance, diagnosis, and grading of this anomaly.Veterinár̆ství 10/2014; 64:739-743.
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ABSTRACT: A 9-year-old Arabian mare was evaluated for a 7-day history of malaise. Results of a CBC included a leukocyte concentration within the reference interval (8.4 x 10(3)/microL, reference interval 6.0-14.0 x 10(3)/microL) with an apparent degenerative left shift (segmented neutrophils 1.2 x 10(3)/microL, reference interval 2.5-7.5 x 10(3)/microL; hyposegmented neutrophils 1.8 x 10(3)/microL, reference interval 0.0-0.2 x 10(3)/microL). Serum clinical chemistry results included increased aspartate transaminase, alkaline phosphatase, and gamma-glutamyltransferase activities. A presumptive diagnosis of hepatitis or cholangiohepatitis was made. The horse was treated with antimicrobials and the malaise quickly resolved. However, in a recheck CBC on day 13, the apparent degenerative left shift remained. Further evaluation of the blood smear revealed many hyposegmented granulocytes with coarse mature chromatin and normal cytoplasmic features. On the basis of the microscopic examination, the horse was diagnosed with Pelger-Huët anomaly. The patient's offspring was subsequently also diagnosed with Pelger-Huët anomaly on the basis of blood film examination. Neutrophil, eosinophil, and basophil mean nuclear scores in both affected horses (mare, range 1.5-2.6; offspring, range 1.6-3.2) were lower than those in 2 unrelated Arabian horses (range, 2.8-5.0) and 5 non-Arabian control horses (range, 2.8-5.0). Results of immunophenotyping and phagocytosis/oxidative burst assays via flow cytometry showed no difference in the expression of myeloid-specific or adhesion molecules or in neutrophil function between affected and control horses. This is the second known report of equine Pelger-Huët anomaly, both of which affected Arabian horses.Veterinary Clinical Pathology 10/2007; 36(3):306-10. · 1.29 Impact Factor
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ABSTRACT: A 13 year old Danish/Swedish Farmdog from Denmark was evaluated in a veterinary clinic in Warsaw, Poland for evaluation of an orthopedic problem. Radiographs revealed spondylosis and degenerative vertebral disease, which responded to treatment with anti-inflammatory medications. A predominance of hyposegmented neutrophils and eosinophils containing condensed chromatin and normal cytoplasm were identified on a routine CBC. Follow-up blood film evaluations over the course of 12 months confirmed that the hyposegmented granulocytes persisted. The majority of neutrophils contained Grade 2 nuclei (slightly indented), and the mean nuclear score varied from 1.9 to 2.3. Pelger-Huët anomaly (PHA), presumably congenital, was diagnosed based on persistent hyposegmented granulocytes in the absence of an underlying cause for acquired PHA; genetically related dogs were unavailable for testing to confirm vertical transmission. To the authors' knowledge this is the first report of PHA in a Danish/Swedish Farmdog.Acta Veterinaria Scandinavica 03/2011; 53:14. · 1.00 Impact Factor