Article

Serovar-specific immune responses to peptides of variable regions of Chlamydia trachomatis major outer membrane protein in serovar D-infected women

Clinical and Experimental Medicine (Impact Factor: 2.4). 12/2008; 8(4):207-215. DOI: 10.1007/s10238-008-0004-2

ABSTRACT The role of major outer membrane protein (MOMP) variable regions in the interaction of chlamydiae and host cells has been
evaluated and their role in neutralization of antibodies has been clearly demonstrated. There are also studies that delineate
the contribution of these regions to the cell-mediated immune response of the host and suggest that serovar E elicits serovar-specific
immune responses in infected humans. However, further studies with other serovars are required to confirm these findings and
to elucidate the role and importance of serovar-specific responses of variable regions of MOMP in other serovars. We, therefore,
performed a detailed analysis of the humoral and cellular immune responses against the serovar D-specific variable segments
(VS) of MOMP in women infected with Chlamydia trachomatis. We found that VS4 elicits significantly higher responses (both humoral and cellular) than other VS peptides (VS1, VS2 and
VS3). VS4 elicited significantly higher (P<0.0001) proliferative responses, interferon-gamma levels (P<0.0001) as well as higher prevalence (P<0.0001) of IgG antibodies against VS4 in serovar D-infected patients as compared to patients infected with other serovars,
suggesting its role in serovar-specific immune responses.

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