Serovar-specific immune responses to peptides of variable regions of Chlamydia trachomatis major outer membrane protein in serovar D-infected women

Clinical and Experimental Medicine (Impact Factor: 2.96). 12/2008; 8(4):207-215. DOI: 10.1007/s10238-008-0004-2


The role of major outer membrane protein (MOMP) variable regions in the interaction of chlamydiae and host cells has been
evaluated and their role in neutralization of antibodies has been clearly demonstrated. There are also studies that delineate
the contribution of these regions to the cell-mediated immune response of the host and suggest that serovar E elicits serovar-specific
immune responses in infected humans. However, further studies with other serovars are required to confirm these findings and
to elucidate the role and importance of serovar-specific responses of variable regions of MOMP in other serovars. We, therefore,
performed a detailed analysis of the humoral and cellular immune responses against the serovar D-specific variable segments
(VS) of MOMP in women infected with Chlamydia trachomatis. We found that VS4 elicits significantly higher responses (both humoral and cellular) than other VS peptides (VS1, VS2 and
VS3). VS4 elicited significantly higher (P<0.0001) proliferative responses, interferon-gamma levels (P<0.0001) as well as higher prevalence (P<0.0001) of IgG antibodies against VS4 in serovar D-infected patients as compared to patients infected with other serovars,
suggesting its role in serovar-specific immune responses.

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    ABSTRACT: Chlamydia trachomatis serovars are divided into three serogroups, namely serogroup B, serogroup I (Intermediate) and serogroup C, and subsequently into 19 different serovars. Worldwide, serogroup B is the most prevalent followed by serogroup I. Clear differences have been observed in the duration of infection and growth kinetics between serovars from different serogroups in murine and cell culture models. Reasons for these observed differences are bacterial and host related, and are not well understood. The aim of this study was to determine the differences in immunoglobulin (Ig) G responses between the three serogroups in a group of patients infected with different serovars. Serovars were assessed from 235 C. trachomatispositive patients and quantitative IgG responses were determined. Analyses of variance were used to compare the IgG responses between the three serogroups. Of the serovars, 46% were B group (with serovar E the most prevalent: 35.3%), 39.6% were I group and 14.3% were C group. A highly significant difference in serologic response was shown when comparing the mean IgG concentrations (AU/mL) of patients having serovars in the most prevalent serogroup compared to the other serogroups: B = 135, C = 46 and I = 60 (B vs. C and B vs. I, P < 0.001). In conclusion, the most prevalent serovars generate the highest serologic responses.
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    ABSTRACT: Chlamydia trachomatis infections are the most common seksual transmitted infections in the Netherlands and worldwide. Many infections run a asymptomatic course, but serious complications may occur, such as PID with subsequent subfertility or ectopic pregancy. For further understanding of the differences in the clinical cousre of infections we determined risk factors in obstetrical and gynaecological patients. We compared various serological test and desctibe the different serovars.
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    ABSTRACT: Based on gold-labeled silver stain (GLSS) method, we developed the visual protein microarray for simultaneous, sensitive, and specific detection of Ureaplasma parvum and Chlamydia trachomatis using N-terminus multiple-banded antigen (NMBA) of U. parvum and major outer membrane protein of C. trachomatis. The specific antigens were immobilized on glass surface that was treated with 3-glycidoxypropyltrimethoxysilane, and they were used as the capturing probes to recognize the complementary target antibodies binding to the detecting probes of Nano-gold-Staphylococcal protein A (SPA). In the "sandwich" format, Nano-gold-SPA probe was used as an indicator and GLSS was applied to amplify the detection signals and produce black image on array spots, which were visible with naked eyes. In our model arrays, the detection limit of protein microarray was as low as 2 ng/mL, and the lowest titer of detectable antibody was 1:128; thus, this sensitivity was comparable to the fluorescent detection method. The visual simultaneous protein microarrays were used to detect total 186 clinical samples, which had been determined by enzyme-linked immunosorbent assay (ELISA) and fluorescence quantitative real-time polymerase chain reaction; the results were identical and no distinct difference (P > 0.05) existed between them. Our results demonstrate that we have developed the visual protein microarray technique, which is of high sensitivity and high specificity, and it may have potential in clinical applications.
    Diagnostic microbiology and infectious disease 03/2010; 67(2):122-8. DOI:10.1016/j.diagmicrobio.2010.01.009 · 2.46 Impact Factor
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