Article

A simplified, colorimetric micromethod for xylose in serum or urine, with phloroglucinol.

Clinical Chemistry (Impact Factor: 7.15). 09/1979; 25(8):1440-3.
Source: PubMed

ABSTRACT We have developed a simplified xylose assay procedure that requires only 10 min and requires 50 microL of serum or 5 microL of urine. The reaction with phloroglucinol is more sensitive than the classic p-bromaniline color reaction, and requires only 4 min of heating for color development. A single reagent is mixed with the specimen directly, without prior protein precipitation. Analytical recovery of xylose added to serum was quantitative; precision studies resulted in a between-day coefficient of variation of 5.2%. Glucose, which has significant potential for interference in most other xylose procedures, reacts under the test conditions only to the extent of 70 mumol of apparent xylose per liter for a 5.5 mmol/L solution of glucose. The new procedure has been valuable in the assessment of malabsorption, especially in children and infants, where serum xylose is the preferred measurement.

0 Bookmarks
 · 
353 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The aim of the present study was to investigate the putative effects of dietary supplementation with green tea powder on growth performance, D-xylose absorption as well as serum-selected parameters of broiler chickens. Forty 21-day-old chickens were randomly allocated into four groups and fed with diets supplemented with 0% (control), 1%, 2%, or 4% green tea powder for 2 weeks. At the end of the experiment, D-xylose absorption test was performed on all birds on a 45-min basis for 135 min, and PCV as well as serum total protein, total cholesterol, LDL-c, HDL-c, triglycerides, iron, calcium, and phosphorus concentrations were assayed. Body weight, feed intake, and feed conver-sion ratio (FCR) were also recorded during the experiment. No significant differences were observed in plasma D-xylose concentrations, PCV, or serum parameters among different groups (p>0.05). Differences in body weight, feed intake, and FCR were insignificant as well. In conclusion, dietary supplementation with green tea powder for 2 weeks has no adverse effect on PCV, intestinal absorption of D-xylose, serum total protein, lipid profile, and mineral concentrations as well as performance parameters of broiler chickens.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Oyster peptides were produced from Crassostrea hongkongensis and used as a new protein source for the preparation of an oyster peptide-based enteral nutrition formula (OPENF). Reserpineinduced malabsorption mice and cyclophosphamide-induced immunosuppression mice were used in this study. OPENF powder is light yellow green and has a protein-fat-carbohydrate ratio of 16:9:75 with good solubility in water. A pilot study investigating immune functional impacts of the OPENF on mice show that the OPENF enhanced spleen lymphocyte proliferation and the activity of natural killer (NK) cells in BALB/c mice. Furthermore, OPENF can improve intestinal absorption, increase food utilization ratio, and maintain the normal physiological function of mice. These results suggest that oyster peptides could serve as a new protein source for use in enteral nutrition formula, but more importantly, also indicate that OPENF has an immunostimulating effect in mice.
    Chinese Journal of Oceanology and Limnology 31(4). · 0.58 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Neonates are at increased risk for inflammatory bowel disease, but effective prevention and treatments are currently limited. This study was conducted with the lipopolysaccharide (LPS)-challenged piglet model to determine the effects of dietary supplementation with α-ketoglutarate (AKG) on the intestinal morphology and function. Eighteen 24-day-old pigs (weaned at 21 days of age) were assigned randomly to control, LPS, and LPS + AKG groups. The piglets in the control and LPS groups were fed a corn- and soybean meal-based diet, whereas the LPS + AKG group was fed the basal diet supplemented with 1% AKG. On days 10, 12, 14, and 16, piglets in the LPS and LPS + AKG groups received intraperitoneal administration of LPS (80 μg/kg BW), whereas piglets in the control group received the same volume of saline. On day 16, d-xylose was orally administrated to all pigs at the dose of 0.1 g/kg BW, 2 h after LPS or saline injection, and blood samples were collected 3 h thereafter. Twenty-four hours post-administration of LPS or saline, pigs were killed to obtain intestinal mucosae for analysis. Compared with the control group, LPS challenge reduced (P
    Amino Acids 01/2010; 39(2):555-564. · 3.91 Impact Factor

Full-text

View
23 Downloads
Available from