Determination of B/A of Biological Media by Measuring and Modeling Nonlinear Distortion of Pulsed Acoustic Wave in Two-Layer System of Media
ABSTRACT Knowledge of the acoustic nonlinearity parameter, B/A, of biological fluids or soft tissues is necessary whenever high intensity pressure fields are induced. A numerical model
recently developed in our lab is capable of fast predicting the nonlinear distortion of pulsed finite-amplitude acoustic waves
generated from axisymmetric sources propagating through multilayer attenuating media. Quantitative analysis of the obtained
results enabled developing the alternative method for determination of the B/A of biological media. First, the method involves measuring the nonlinear waveform distortion of the tone burst propagating
through water. Then, it involves numerical modeling (in frequency domain) using the Time-Averaged Wave Envelope (TAWE) approach.
The numerical simulation results are fitted to the experimental data by adjusting the source boundary conditions to determine
accurately the source pressure, effective radius and apodization function being the input parameters to the numerical solver.
Next, the method involves measuring the nonlinear distortion of idem tone burst passing through the two-layer system of parallel
media. Then, we numerically model nonlinear distortion in two-layer system of media in frequency domain under experimental
boundary conditions. The numerical simulation results are fitted to the experimental data by adjusting the B/A value of the tested material. Values of the B/A for 1.3-butanediol at both the ambient (25°C) and physiological (36.6°C) temperatures were determined. The obtained result (B/A = 10.5 ± 5% at 25°C) is in a good agreement with that available in literature. The B/A = 11.5 ± 5% at 36.6°C was determined.
KeywordsNonlinearity parameter measurement-
-Nonlinear propagation-Biological media-PVDF membrane hydrophone
Full-textDOI: · Available from: Janusz Wójcik, Oct 14, 2014
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- "Then the focal plane occurs within the tissue sample. In previous publications   have been shown that for any circular transducer with ka >>1 and weak to moderate source pressure level the axial distance z 1 at which sudden growth of the second harmonics begins (for the tone burst generated from that transducer in water and propagating there) is specific for that transducer and constant independently on the source pressure amplitude. The weak to moderate source level means that in the nonlinear acoustic field produced by the source in water the ratio of the shock formation distance l D to the Rayleigh distance R 0 is larger than about 0.3 . "
ABSTRACT: Beneficial biological effects in soft tissues can be induced by focused ultrasound of low intensity (LIFU). For example, increasing of cells immunity to stress can be accomplished through the enhanced heat shock proteins (Hsp) expression induced by the low intensity focused ultrasound. The possibility to control the Hsp expression enhancement in soft tissues in vivo can be the potential new therapeutic approach to neurodegenerative diseases that utilizes the known feature of cells to increase their immunity to stresses through the Hsp expression enhancement. The controlling of the Hsp expression enhancement by adjusting the level of exposure to ultrasound energy would allow evaluating of ultrasound-mediated treatment efficiency. Our objective was to develop the numerical model capable of predicting in space and time temperature fields induced in multilayer nonlinear attenuating media by a circular focused transducer generating pulsed acoustic waves and to compare the results calculated for two-layer configuration of media: water -fresh rat liver with the experimental data. The measurements of temperature variations versus time at 5 points on the acoustic beam axis within the tissue sample were performed using 0.2-mm diameter thermocouples. Temperature fields were induced by the transducer with 15-mm diameter, 25-mm focal length and 2-MHz centre frequency generating tone bursts with the intensity I SPTA varied between 0.45 W/cm 2 and 1.7 W/cm 2 and duration varied between 20 and 500 cycles at the same 20-% duty cycle and 20-min exposure time. Quantitative analysis of the obtained results allowed to show that, for example, for the acoustic beam with intensity I SPTA = 1.13 W/cm 2 exposure time to ultrasound should not be longer than 10 min to avoid cells necrosis following the 43-o C temperature threshold exceeding.
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ABSTRACT: Recent research has shown that beneficial therapeutic effects in soft tissues can be induced by the low power ultrasound (LPUS). For example, increasing of cells immunity to stress (among others thermal stress) can be obtained through the enhanced heat shock proteins (Hsp) expression induced by the low intensity ultrasound. The possibility to control the Hsp expression enhancement in soft tissues in vivo stimulated by ultrasound can be the potential new therapeutic approach to the neurodegenerative diseases which utilizes the known feature of cells to increase their immunity to stresses through the Hsp expression enhancement. The controlling of the Hsp expression enhancement by adjusting of exposure level to ultrasound energy would allow to evaluate and optimize the ultrasound-mediated treatment efficiency. Ultrasonic regimes are controlled by adjusting the pulsed ultrasound waves intensity, frequency, duration, duty cycle and exposure time. Our objective was to develop the numerical model capable of predicting in space and time temperature fields induced by a circular focused transducer generating tone bursts in multilayer nonlinear attenuating media and to compare the numerically calculated results with the experimental data in vitro. The acoustic pressure field in multilayer biological media was calculated using our original numerical solver. For prediction of temperature fields the Pennes' bio-heat transfer equation was employed. Temperature field measurements in vitro were carried out in a fresh rat liver using the 15 mm diameter, 25 mm focal length and 2 MHz central frequency transducer generating tone bursts with the spatial peak temporal average acoustic intensity varied between 0.325 and 1.95 W/cm2, duration varied from 20 to 500 cycles at the same 20% duty cycle and the exposure time varied up to 20 minutes. The measurement data were compared with numerical simulation results obtained under experimental boundary conditions. Good agreement between the theoretical and measurement results for all cases considered has verified the validity and accuracy of our numerical model. Quantitative analysis of the obtained results enabled to find how the ultrasound-induced temperature rises in the rat liver could be controlled by adjusting the source parameters and exposure time.03/2010; DOI:10.1063/1.3367179
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ABSTRACT: Many therapeutic applications of pulsed focused ultrasound are based on heating of detected lesions which may be localized in tissues at different depths under the skin. In order to concentrate the acoustic energy inside tissues at desired depths a new approach using a planar multi-element annular array transducer with an electronically adjusted time-delay of excitation of its elements, was proposed. The 7-elements annular array transducer with 2.4 MHz center operating frequency and 20 mm outer diameter was produced. All its elements (central disc and 6 rings) had the same radiating area. The main purpose of this study was to investigate ther-mal fields induced in bovine liver in vitro by pulsed focused ultrasonic beams with various acoustic properties and electronically steered focal plane generated from the annular array transducer used. The measurements were performed for the radiating beams with the 20 mm focal depth. In order to maximize nonlinear effects introduc-ing the important local temperature rise, the measurements have been performed in two-layer media comprising of a water layer, whose thickness was specific for the transducer used and equal to 13 mm, and the second layer of a bovine liver with a thickness of 20 mm. The thickness of the water layer was determined numerically as the axial distance where the amplitude of the second harmonics started to increase rapidly. The measurements of the temperature rise versus time were performed us-ing a thermocouple placed inside the liver at the focus of the beam. The temperature rise induced in the bovine liver in vitro by beams with the average acoustic power of 1 W, 2 W and 3 W and duty cycle of 1/5, 1/15 and 1/30, respectively, have been mea-sured. For each beam used the exposure time needed for the local tissue heating to the temperature of 43 • C (used in therapies based on ultrasonic enhancement of drug delivery or in therapies involving stimulation of immune system by enhancement of the heat shock proteins expression) and to the temperature of 56 • C (used in HIFU therapies) was determined. Two sets of measurements were done for each beam con-sidered. First, the thermocouple measurement of the temperature rise was done and 938 T. Kujawska et al. next, the real-time monitoring of dynamics of growth of the necrosis area by using ultrasonic imaging technique, while the sample was exposed to the same acoustic beam. It was found that the necrosis area becomes visible in the ultrasonic image only for beams with the average acoustic power of 3 W, although after cutting the sample the thermo ablated area was visible with the naked eye even for the beams with lower acoustic power. The quantitative analysis of the obtained results allowed to determine the exposure time needed to get the necrosis area visible in the ultrasonic image.Archives of Acoustics 01/2011; 36(4). DOI:10.2478/v10168-011-0063-3 · 0.66 Impact Factor