LC Enantioseparation of β-Amino Acids on a Crown Ether-Based Stationary Phase

Chromatographia (Impact Factor: 1.37). 10/2008; 68:13-18. DOI: 10.1365/s10337-007-0498-x

ABSTRACT Reversed-phase high-performance liquid chromatographic methods were developed for the enantioseparation of ten unusual β-3-homo-amino
acids. The underivatized analytes were separated on a chiral stationary phase containing (+)-(18-crown-6)-2,3,11,12-tetracarboxylic
acid as chiral selector. The effects of organic and acidic modifiers and the mobile phase composition on the separation were
investigated. The structures of the substituents in the β position substantially influenced the retention and resolution.
The elution sequence was determined in some cases: the S enantiomers eluted before the R enantiomers.

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Oct 10, 2014