Article

Copper(II) complexes of diclofenac: Spectroscopic studies and DNA strand breakage

University of Ioannina; Aristotle University of Thessaloniki
BioMetals (impact factor: 2.82). 05/1999; 12(2):167-172. DOI:10.1023/A:1009246122577 pp.167-172

ABSTRACT Copper(II) complexes of diclofenac with interesting anti-inflammatory profiles have been prepared and studied by infrared and electronic spectroscopy. In the solid state and in polar and coordinating solvents, all the complexes are solvated binuclear carboxylato-bridged complexes, [Cu(L)2(S)]2, where L is monodeprotonated diclofenac and S is the axially bonded solvent. The effect of the copper(II) complexes on the in vitro DNA strand breakeage was studied by agarose gel electrophoresis. Relaxation or double stranded scissions of pDNA were observed leading to the formation of linear pDNA. Treatment of pDNA with high concentrations of these compounds caused a disappearance of pDNA. For the parent drug, sodium diclofenac, no effect on the pDNA was observed. This study presents some indications that the binuclear copper(II) complexes, [Cu(L)2(S)]2, could have some relevance in the treatment of tumor cell lines.

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    Article: In vitro efficacy of diclofenac against Listeria monocytogenes.
    N K Dutta, K Mazumdar, M W Baek, D J Kim, Y R Na, S H Park, H K Lee, B H Lee, J H Park
    [show abstract] [hide abstract]
    ABSTRACT: Chemotherapy is often futile in systemic listeriosis, translating to being a peril to public health. There is, thus, an imperative need for novel antilisterial compounds, possibly acting through mechanisms dissimilar to those of existing drugs. The present study describes one such agent-the non-steroidal anti-inflammatory drug (NSAID) diclofenac sodium (Dc). The National Committee for Clinical Laboratory Standards (NCCLS) minimum inhibitory concentration (MIC), mode of action, and two mechanisms of action, i.e., on bacterial DNA and membrane, have been characterized with respect to Dc. The drug showed noteworthy inhibitory action (MIC90 = 50 microg/ml) against Listeria strains, demonstrated cidal (minimum bactericidal concentration [MBC]=100 microg/ml) activity, inhibited listerial DNA synthesis (45.48%; incorporation of [methyl-3H] thymidine), and possessed bacterial membrane-damaging activity (37.33%; BacLight assay). Dc could be used as a lead compound for the synthesis of new, more active agents perhaps devoid of side effects. Further, quantitative structure-activity relationship (QSAR) studies will contribute to a new generation of promising adjuvants to existing antilisterial drugs.
    European Journal of Clinical Microbiology 05/2008; 27(4):315-9. · 2.86 Impact Factor
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Keywords

agarose gel electrophoresis
 
axially
 
binuclear copper(II)
 
Copper(II)
 
disappearance
 
electronic spectroscopy
 
interesting anti-inflammatory profiles
 
linear pDNA
 
pDNA
 
polar
 
scissions
 
sodium diclofenac
 
study presents
 
vitro DNA strand breakeage