RNA interference and its therapeutic potential

Central European Journal of Medicine (Impact Factor: 0.26). 01/2011; 6(2):137-147. DOI: 10.2478/s11536-011-0005-5

ABSTRACT RNA interference is a technique that has become popular in the past few years. This is a biological method to detect the activity
of a specific gene within a cell. RNAi is the introduction of homologous double stranded RNA to specifically target a gene’s
product resulting in null or hypomorphic phenotypes. This technique involves the degradation of specific mRNA by using small
interfering RNA. Both microRNA (miRNA) and small interfering RNA (siRNA) are directly related to RNA interference. RNAi mechanism
is being explored as a new technique for suppressing gene expression. It is an important issue in the treatment of various
diseases. This review considers different aspects of RNAi technique including its history of discovery, molecular mechanism,
gene expression study, advantages of this technique against previously used techniques, barrier associated with this technique,
and its therapeutic application.

KeywordsCancer–MicroRNA–Interfering RNA–Disease

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    ABSTRACT: Head and neck squamous cell cancer (HNSCC) is the six most common cancer in the world. In attempts to improve therapy of this cancer, contemporary research is focused predominantly on new tumor markers and evaluation of their diagnostic, prognostic and predictive significance for routine clinical practice. Molecular tumor markers at the same time very well reflect pathobiological processes in tumors. New etiological and pathobiological aspects of cancerogenesis of HNSCC were lately introduced. Namely epigenetic mechanisms, RNA interference and microRNA, cancer stem cells and epithelial-mesenchymal transition play important role in cancerogenesis as well as represent new source of molecular tumor markers and potential target of biological therapy.
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May 23, 2014