Shear stress effects on plant cell suspension cultures in a rotating wall vessel bioreactor

Journal of Industrial Microbiology and Biotechnology (Impact Factor: 2.51). 12/1998; 22(1):44-47. DOI: 10.1038/sj.jim.2900600

ABSTRACT A rotating wall vessel, designed for growth of mammalian cells under microgravity, was used to study shear effects on Taxus cuspidata plant suspension cell cultures. Shear stress, as quantified by defined shear fields of Couette viscometers, improved specific
cell growth rates and was detrimental to volumetric product formation rates.

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Oil palm tissue culture offers a potentially practical route to clonal propagation of high yielding palms. However, current tissue culture methods are laborious and costly, and the performance of the cultures can be difficult to describe quantitatively. Computer control of bioreactor processes increases reproducibility and permits quantitative description of the growth of oil palm cultures. Even so, there remain unmet needs in the areas of online metabolite measurement and of automation of the tissue culture process. In this work, we apply Raman spectroscopy for non-destructive off-line quantitation of sucrose, glucose, fructose, nitrate, potassium phosphate and magnesium sulphate metabolites in oil palm bioreactor culture supernatants. We also explore the feasibility of using fluorescence to discriminate between different morphotypes of oil palm calli. Finally, we report the use of flow cytometry to sort oil palm suspension cultures on the basis of size; selected samples were deposited into separate wells in a microplate with one callus particle per well. The technologies described in this article contribute to the development of automated methods for moving and positioning oil palm cells, and for online measurement of metabolites in oil palm bioreactor supernatant.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Plant cell culture is traditionally viewed as a unique artifi cially created biological system representing a heterogenous population of dedifferentiated cells. This system undergoes a continuous process of autoselection based on the intensity and stability of cell proliferation. We discuss here the details of formation and regulation of isoprenoid biosynthesis in plant cell in vitro based on literature survey and our research. Obviously, secondary metabolism differs in cell culture compared to the plant per se , because in cell culture metabolites are synthesized and compart-mentalized within a single heterotrophic cell with sparse or underdeveloped vacu-oles and plastids. For example, in plant cell cultures isoprenoid biosynthesis via MVA pathway was found to be more active than via plastid-localized MEP pathway. Also, it was hypothesized that cell cultures preferably produce metabolites, which promote cell proliferation and growth. Indeed, cell cultures of Dioscorea deltoidea produced mainly furostanol glycosides, which promoted cell division. Triterpene glycosides (ginsenosides) in the cell cultures of various Panax species are represented mainly by Rg-and Rb-groups. Rb ginsenosides are predominantly found as malonyl-esters that may infl uence their intracellular localization. Despite the difference in the isoprenoid composition in plant and cell culture the latter became an attractive source of phytochemicals as an alternative to plant harvesting. We provide in this chapter the guidelines to biotechnological production of plant isoprenoids using plant cell cultures and discuss the optimal methods of bioreactor-based cultivation and cryopreservation of plant cell collections.
    Production of Biomass and Bioactive Compounds Using Bioreactor Technology, Edited by K.-Y. Paek et al, 01/2014: chapter Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors: pages 563-623; Springer Science+Business Media Dordrecht.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Salidroside and its aglycone tyrosol are important compounds found in Rhodiola plants. In this study, callus derived from Rhodiola crenulata was induced and grown when explants were incubated on a Murashige and Skoog (MS) medium containing various concentrations of 6-benzyaldenine (BA), naphthalene acetic acid (NAA) and thidiazuron (TDZ). Callus was easily initiated from juvenile leaves in half strength MS medium supplemented with 0.5 mg/L BA and 3.0 mg/L NAA, while full strength MS containing 0.5 mg/L TDZ and 0.5 mg/L NAA was the best for callus subculture and subsequent cell suspension culture. The activities of l-phenylalanine ammonia lyase (PAL) and β-d-glucosidase, two key enzymes in salidroside synthesis, increased at first and subsequently decreased in cell suspension cultures. The salidroside and tyrosol levels in the cell suspension cultures were determined using high-performance liquid chromatography. High levels of salidroside and tyrosol were detected in cell suspension cultures of R. crenulata extracted with 75 % methanol, demonstrating that the biotechnological production of these compounds using plant cell suspension cultures derived from R. crenulata may be an attractive alternative to harvest-based production.
    Plant Cell Tissue and Organ Culture 09/2013; 114(3). DOI:10.1007/s11240-013-0325-z · 2.61 Impact Factor