Shear stress effects on plant cell suspension cultures in a rotating wall vessel bioreactor

Journal of Industrial Microbiology and Biotechnology (Impact Factor: 2.44). 12/1998; 22(1):44-47. DOI: 10.1038/sj.jim.2900600


A rotating wall vessel, designed for growth of mammalian cells under microgravity, was used to study shear effects on Taxus cuspidata plant suspension cell cultures. Shear stress, as quantified by defined shear fields of Couette viscometers, improved specific
cell growth rates and was detrimental to volumetric product formation rates.

Download full-text


Available from: James C Linden, Oct 13, 2015
70 Reads
  • [Show abstract] [Hide abstract]
    ABSTRACT: Salidroside and its aglycone tyrosol are important compounds found in Rhodiola plants. In this study, callus derived from Rhodiola crenulata was induced and grown when explants were incubated on a Murashige and Skoog (MS) medium containing various concentrations of 6-benzyaldenine (BA), naphthalene acetic acid (NAA) and thidiazuron (TDZ). Callus was easily initiated from juvenile leaves in half strength MS medium supplemented with 0.5 mg/L BA and 3.0 mg/L NAA, while full strength MS containing 0.5 mg/L TDZ and 0.5 mg/L NAA was the best for callus subculture and subsequent cell suspension culture. The activities of l-phenylalanine ammonia lyase (PAL) and β-d-glucosidase, two key enzymes in salidroside synthesis, increased at first and subsequently decreased in cell suspension cultures. The salidroside and tyrosol levels in the cell suspension cultures were determined using high-performance liquid chromatography. High levels of salidroside and tyrosol were detected in cell suspension cultures of R. crenulata extracted with 75 % methanol, demonstrating that the biotechnological production of these compounds using plant cell suspension cultures derived from R. crenulata may be an attractive alternative to harvest-based production.
    Plant Cell Tissue and Organ Culture 09/2013; 114(3). DOI:10.1007/s11240-013-0325-z · 2.13 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Oil palm tissue culture offers a potentially practical route to clonal propagation of high yielding palms. However, current tissue culture methods are laborious and costly, and the performance of the cultures can be difficult to describe quantitatively. Computer control of bioreactor processes increases reproducibility and permits quantitative description of the growth of oil palm cultures. Even so, there remain unmet needs in the areas of online metabolite measurement and of automation of the tissue culture process. In this work, we apply Raman spectroscopy for non-destructive off-line quantitation of sucrose, glucose, fructose, nitrate, potassium phosphate and magnesium sulphate metabolites in oil palm bioreactor culture supernatants. We also explore the feasibility of using fluorescence to discriminate between different morphotypes of oil palm calli. Finally, we report the use of flow cytometry to sort oil palm suspension cultures on the basis of size; selected samples were deposited into separate wells in a microplate with one callus particle per well. The technologies described in this article contribute to the development of automated methods for moving and positioning oil palm cells, and for online measurement of metabolites in oil palm bioreactor supernatant.
  • [Show abstract] [Hide abstract]
    ABSTRACT: The present review describes the influence of different types of mixing systems under excess turbulence conditions on microorganisms. Turbohypobiosis phenomena were described by applying a method for measurement of the kinetic energy of flow fluctuations based on the piezoeffect. It can be assumed that the shear stress effect (the state of turbohypobiosis) plays a role mainly when alternative mechanisms in cells cannot ensure a normal physiological state under stress conditions. Practically any system (inner construction of a bioreactor, culture and cultivation conditions, including mixing) requires its own optimisation to achieve the final goal, namely, the maximum product and/or biomass yields from substrate (Y P/S or/and Y X/S ), respectively. Data on the biotechnological performance of cultivation as well as power input, kinetic energy (e) of flow fluctuations, air consumption rate, rotational speed, tip speed, etc. do not correlate directly if the mixing systems (impellers-baffles) are dissimilar. Even the widely used specific power consumption cannot be relied upon for scaling up the cultivation performance using dissimilar mixing systems. A biochemical explanation for substrate and product transport via cell walls, carbon pathways, energy generation and utilisation, etc. furnishes insight into cellular interactions with turbulence of different origin for different types of microorganisms (single cells, mycelia forming cells, etc.).
    Central European Journal of Biology 12/2007; 2(4):481-501. DOI:10.2478/s11535-007-0038-6 · 0.71 Impact Factor
Show more