Comparative study of estradiol trapped in polymer gels versus estradiol conjugated to bovine serum albumin for production of polyclonal antibody

Journal of Radioanalytical and Nuclear Chemistry (Impact Factor: 1.03). 08/2011; 289(2):647-652. DOI: 10.1007/s10967-011-1133-8

ABSTRACT The aim of this work is the development of the immunogenic properties of 17beta-estradiol to produce polyclonal antibody for
radioimmunoassay purposes. Poly(acrylamide–maleic acid) gel [P(AAm–MA)] was prepared by the template polymerization. Polyacrylamide
(PAAm) was used as a template for the polymerization of MA in aqueous solution using gamma rays as initiator. Then a comparative
study of estradiol trapped in the prepared polymer gels versus estradiol conjugated to bovine serum albumin (BSA) was carried
out. Ten rats divided into two groups were injected according to specific protocol. The immunogenic properties of both produced
polyclonal antibodies were studied. In conclusion, the results revealed that the similar immunogenic properties with specific
radiolabeled tracer were obtained.

KeywordsPolyclonal antibody–Estradiol–Gel–Template polymerization–RIA

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