Isolation and characterization of 16 polymorphic microsatellite markers from Nibea albiflora

Abstract

Nibea albiflora is a commercially important fish species in China. Herein we report 16 novel polymorphic microsatellite markers in Nibea albiflora by using the 5′ anchored polymerase chain reaction (PCR) technique. The characteristics of these loci were estimated by using
a sample of 30 individuals. A total of 79 alleles were detected with an average of 4.9 alleles per locus. The number of alleles
per locus ranged from three to nine. The polymorphism information content (PIC) values for the 16 microsatellite loci ranged
from 0.3131 to 0.7910. The observed and expected heterozygosity per locus ranged from 0.2333 to 1.000 and from 0.3452 to 0.8421,
with an average of 0.7248 and 0.6592, respectively. Four loci significantly deviated from Hardy–Weinberg equilibrium after
Bonferroni correction (P<0.0031), and no significant linkage disequilibrium between pairs of loci was found. This study provides useful information
for studies on genetic diversity and structure, construction of genetic linkage maps of N.albiflora, and effective management of this fish resource.

Keywords
Nibea albiflora
–Microsatellite markers–Polymorphism–5′ Anchored PCR