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Biocompatibility of FGL peptide self-assembly nanofibers with neural stem cells in vitro

Journal of Wuhan University of Technology-Mater Sci Ed (Impact Factor: 0.48). 24(6):992-996. DOI: 10.1007/s11595-009-6992-2

ABSTRACT In order to study the biocompatibility of self-assembled FGL peptide nanofibers scaffold with neural stem cells (NSCs), FGL
pepitide-amphiphile (FGL-PA) was synthesized by solid-phase peptide synthesis technique. The diluted hydrochloric acid was
added into FGL-PA solution to reduce the PH value and accordingly induce self-assembly. The morphological features of the
assembled material were studied by transmission electron microscope. NSCs were cultured and added with self-assembled FGL-PA.
CCK-8 kit was used to test its effect on the proliferation of NSCs. The differentiation of NSCs was also tested after FGL-PA
assembled material added. The experimental results showed that FGL-PA could be self-assembled to form a hydrogel. TEM analysis
showed the self-assembled hydrogel was nanofibers with diameter of 10–20 nm and length of hundreds nanometers. FGL-PA with
concentrations of 50,100, or 200 mg/L could promote the proliferation of NSCs, and absorbance of them was increased (P<0.05). The rate of neurons differentiated from NSCs was improved greatly by FGL-PA assembled material compared with control
(P<0.05). The findings suggested that FGL-PA could self-assemble to nanofiber hydrogel, which had good biocompatibility with
NSCs.

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