Host range expansion of honey bee Black Queen Cell Virus in the bumble bee, Bombus huntii

Apidologie (Impact Factor: 1.68). 09/2011; 42(5):650-658. DOI: 10.1007/s13592-011-0061-5


Here we provide the first evidence that Black Queen Cell Virus (BQCV), one of the most prevalent honey bee viruses, can cause
an infection in bumble bees, Bombus huntii, and that the BQCV infection could spread to different tissues of bumble bees. The detection of negative strand RNA of BQCV,
an indicator of active virus replication, in the gut of B. huntii suggests that virus particles replicate within the gut and then cross the gut lining to other tissues through hemolymph circulation.
The observation of active replication of the BQCV in the gut, together with the fact that BQCV was more widespread in the
body of field-collected bees than that of lab-reared bees, implies a possible association between the foraging activities
of bumble bees and virus transmission. The fact that bumble bees and honey bees are able to share nectar and pollen resources
in the same field suggests that geographical proximity of two host species could play a role in host range breadth of BQCV.

Keywordshost range–bumble bee–
Bombus huntii
–Black queen cell virus

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Available from: Jilian Li, Apr 17, 2015
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    • "Other than detecting honeybee pathogens in 110 other insects, and thus establishing possible transmission routes 111 (e.g. (Evison et al., 2012; Li et al., 2011; Peng et al., 2011; Singh 112 et al., 2010), there has been little research as to whether these 113 viruses are actually infectious or, more importantly, cause damage 114 to species other than honeybees. The only recorded exceptions so 115 far are the association of DWV with wing deformities found natu- 116 rally in both wild and commercially reared bumblebees (Genersch 117 et al., 2006), the reduced survival of bumblebees orally inoculated 118 with DWV (Fürst et al., 2014) and the rapid mortality of bumble- 119 bees injected with low doses of Israeli acute paralysis virus (IAPV; "
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    ABSTRACT: Israeli acute paralysis virus (IAPV) together with Acute bee paralysis virus (ABPV) and Kashmir bee virus (KBV) constitute a complex of closely related dicistroviruses. They are infamous for their high mortality after injection in honeybees. These viruses have also been reported in non-Apis hymenopteran pollinators such as bumblebees, which got infected with IAPV when placed in the same greenhouse with IAPV infected honeybee hives. Here we orally infected Bombus terrestris workers with different doses of either IAPV or KBV viral particles. The success of the infection was established by analysis of the bumblebees after the impact studies: 50 days after infection. Doses of 0.5 x 10(7) and 1 x 10(7) virus particles per bee were infectious over this period, for IAPV and KBV respectively, while a dose of 0.5 x 10(6) IAPV particles per bee was not infectious. The impact of virus infection was studied in micro-colonies consisting of 5 bumblebees, one of which becomes a pseudo-queen which proceeds to lay unfertilized (drone) eggs. The impact parameters studied were: the establishment of a laying pseudo-queen, the timing of egg-laying, the number of drones produced, the weight of these drones and worker mortality. In this setup KBV infection resulted in a significant slower colony startup and offspring production, while only the latter can be reported for IAPV. Neither virus increased worker mortality, at the oral doses used. We recommend further studies on how these viruses transmit between different pollinator species. It is also vital to understand how viral prevalence can affect wild bee populations because disturbance of the natural host-virus association may deteriorate the already critically endangered status of many bumblebee species.
    Journal of Invertebrate Pathology 07/2014; 121. DOI:10.1016/j.jip.2014.06.011 · 2.11 Impact Factor
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    • "In a previous study we reported the detection of deformed wing virus (DWV), black queen cell virus (BQCV), Israeli acute paralysis virus (IAPV), Kashmir bee virus (KBV), and sacbrood virus (SBV) in 11 non-Apis hymenopteran species and in pollen pellets from forager bees (Singh et al., 2010). Other studies have also identified DWV and BQCV infections in some species of bumble bees, including Bombus terrestris, Bombus pascuorum, and Bombus huntii (Genersch et al., 2006; Li et al., 2011; Meeus et al., 2010; Morkeski and Averill, 2010; Peng et al., 2011). Other viruses normally found in A. mellifera, including acute bee paralysis virus (ABPV) and KBV, have also been identified in bumble bees (Anderson, 1991; Bailey and Gibbs, 1964). "
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    ABSTRACT: There are a number of RNA virus pathogens that represent a serious threat to the health of managed honey bees (Apis mellifera). That some of these viruses are also found in the broader pollinator community suggests the wider environmental spread of these viruses, with the potential for a broader impact on ecosystems. Studies on the ecology and evolution of these viruses in the arthropod community as a whole may therefore provide important insights into these potential impacts. We examined managed A. mellifera colonies, nearby non-Apis hymenopteran pollinators, and other associated arthropods for the presence of five commonly occurring picorna-like RNA viruses of honey bees-black queen cell virus, deformed wing virus, Israeli acute paralysis virus, Kashmir bee virus and sacbrood virus. Notably, we observed their presence in several arthropod species. Additionally, detection of negative-strand RNA using strand-specific RT-PCR assays for deformed wing virus and Israeli acute paralysis virus suggests active replication of deformed wing virus in at least six non-Apis species and active replication of Israeli acute paralysis virus in one non-Apis species. Phylogenetic analysis of deformed wing virus also revealed that this virus is freely disseminating across the species sampled in this study. In sum, our study indicates that these viruses are not specific to the pollinator community and that other arthropod species have the potential to be involved in disease transmission in pollinator populations.
    Virus Research 07/2013; 176(1-2). DOI:10.1016/j.virusres.2013.06.013 · 2.32 Impact Factor
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    • "For instance, Melissococcus plutonius, a bacterium that infects A. mellifera larvae leading to European foulbrood disease, caused serious damage to colonies of A. cerana in China from 1972 to 1976 [17]. Further, viruses that are frequent in A. mellifera have been identified in A. cerana [25]–[26] and different species of bumble bees [27]–[29], as well as in eleven non-apis hymenopteran species [30]. On the other hand, V. destructor was transmitted from its original host A. cerana to A. mellifera in the middle of the 20th century [31] and has caused catastrophic damage to A. mellifera since then. "
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    ABSTRACT: Pathogens and parasites represent significant threats to the health and well-being of honeybee species that are key pollinators of agricultural crops and flowers worldwide. We conducted a nationwide survey to determine the occurrence and prevalence of pathogens and parasites in Asian honeybees, Apis cerana, in China. Our study provides evidence of infections of A. cerana by pathogenic Deformed wing virus (DWV), Black queen cell virus (BQCV), Nosema ceranae, and C. bombi species that have been linked to population declines of European honeybees, A. mellifera, and bumble bees. However, the prevalence of DWV, a virus that causes widespread infection in A. mellifera, was low, arguably a result of the greater ability of A. cerana to resist the ectoprasitic mite Varroa destructor, an efficient vector of DWV. Analyses of microbial communities from the A. cerana digestive tract showed that Nosema infection could have detrimental effects on the gut microbiota. Workers infected by N. ceranae tended to have lower bacterial quantities, with these differences being significant for the Bifidobacterium and Pasteurellaceae bacteria groups. The results of this nationwide screen show that parasites and pathogens that have caused serious problems in European honeybees can be found in native honeybee species kept in Asia. Environmental changes due to new agricultural practices and globalization may facilitate the spread of pathogens into new geographic areas. The foraging behavior of pollinators that are in close geographic proximity likely have played an important role in spreading of parasites and pathogens over to new hosts. Phylogenetic analyses provide insights into the movement and population structure of these parasites, suggesting a bidirectional flow of parasites among pollinators. The presence of these parasites and pathogens may have considerable implications for an observed population decline of Asian honeybees.
    PLoS ONE 11/2012; 7(11):e47955. DOI:10.1371/journal.pone.0047955 · 3.23 Impact Factor
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