Caldicoprobacteralgeriensis sp. nov. a New Thermophilic Anaerobic, Xylanolytic Bacterium Isolated from an Algerian Hot Spring

Current Microbiology (Impact Factor: 1.42). 03/2011; 62(3):826-832. DOI: 10.1007/s00284-010-9789-9


A thermophilic anaerobic bacterium (strain TH7C1T) was isolated from the hydrothermal hot spring of Guelma in the northeast of Algeria. Strain TH7C1T stained Gram-positive, was a non-motile rod appearing singly, in pairs, or as long chains (0.7–1×2–6μm2). Spores were never observed. It grew at temperatures between 55 and 75°C (optimum 65°C) and at pH between 6.2 and 8.3 (optimum
6.9). It did not require NaCl for growth, but tolerated it up to 5gl−1. Strain TH7C1T is an obligatory heterotroph fermenting sugars including glucose, galactose, lactose, raffinose, fructose, ribose, xylose,
arabinose, maltose, mannitol, cellobiose, mannose, melibiose, saccharose, but also xylan, and pyruvate. Fermentation of sugars
only occurred in the presence of yeast extract (0.1%). The end-products from glucose fermentation were acetate, lactate, ethanol,
CO2, and H2. Nitrate, nitrite, thiosulfate, elemental sulfur, sulfate, and sulfite were not used as electron acceptors. The G+C content
of the genomic DNA was 44.7mol% (HPLC techniques). Phylogenetic analysis of the small-subunit ribosomal RNA (rRNA) gene sequence
indicated that strain TH7C1T was affiliated to Firmicutes, order Clostridiales, family Caldicoprobacteraceae, with Caldicoprobacter
oshimai (98.5%) being its closest relative. Based on phenotypic, phylogenetic, and genetic characteristics, strain TH7C1T is proposed as a novel species of genus Caldicoprobacter, Caldicoprobacter algeriensis, sp. nov. (strain TH7C1T=DSM 22661T=JCM 16184T).

50 Reads
  • Source
    • "The biological study of these ecosystems in Algeria started more than 74 years ago with the expedition of a French colonial explorer who studied the fauna of one of the hottest springs, ''Meskoutine spring'' (Masson 1939). However, only a few studies on thermophilic bacteria inhabiting these hot springs, with the isolation of new extremophile species have been conducted since then (Kecha et al. 2007; Bouanane-Darenfed et al. 2011), and the cyanobacteria of Algerian hot springs have remained unexplored so far. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Geothermal springs in Algeria have been known since the Roman Empire. They mainly locate in Eastern Algeria and are inhabited by thermophilic organisms, which include cyanobacteria forming mats and concretions. In this work, we have investigated the cyanobacterial diversity of these springs. Cyanobacteria were collected from water, concretions and mats in nine hot springs with water temperatures ranging from 39 to 93 °C. Samples were collected for isolation in culture, microscopic morphological examination, and molecular diversity analysis based on 16S rRNA gene sequences. Nineteen different cyanobacterial morphotypes were identified, the most abundant of which were three species of Leptolyngbya, accompanied by members of the genera Gloeocapsa, Gloeocapsopsis, Stigonema, Fischerella, Synechocystis, Microcoleus, Cyanobacterium, Chroococcus and Geitlerinema. Molecular diversity analyses were in good general agreement with classical identification and allowed the detection of additional species in three springs with temperatures higher than 50 °C. They corresponded to a Synechococcus clade and to relatives of the intracellularly calcifying Candidatus Gloeomargarita lithophora. The hottest springs were dominated by members of Leptolyngbya, Synechococcus-like cyanobacteria and Gloeomargarita, whereas Oscillatoriales other than Leptolyngbya, Chroococcales and Stigonematales dominated lower temperature springs. The isolation of some of these strains sets the ground for future studies on the biology of thermophilic cyanobacteria.
    Extremophiles 07/2014; 18(6). DOI:10.1007/s00792-014-0680-7 · 2.31 Impact Factor
  • Source
    • "ing waters for human health risk (Marovic et al. 1996; Singh 2014). Algerian thermal springs have been studied mostly from the perspective of geothermal resources (Kedaid 2007; Saibi 2009), on hydrogeological aspects and physicochemical composition (Verdeil 1982; Dib 1985; Issaadi 1992), as well as for the first radon content studies which started more than 85 years ago with the expedition of the French colonial explorers (Pouget and Chouchak 1923) and few recent studies on thermophilic bacteria inhabiting hot springs and isolation of new extremophile species (Kecha et al. 2007; Bouanane-Darenfed et al. 2011). But none of them concentrate on the monitoring studies of their radiological and bacteriological contents. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Hot springs have been known for a long time over the world. They are used for curative action and healing properties linked to their physical and chemical characteristics. In order to best use the opportunities provided by globalization, Algerian authorities try to modernize its social and economic policies investing mainly in geothermal resources recognizing that the hot springs have rich potential to develop ecotourism. Nevertheless, the use of thermal waters requires nowadays a prior characterization to study their quality. The mineral bacteriology assessment Type 0, Type 1, and Type 2 are used to assess the microbiological quality. It consists of the enumeration of total aerobic flora (37 \(^{\circ }\hbox {C})\) , Coliforms and Escherichia coli, intestinal enterococci, sulphite-reducing bacteria spores, Pseudomonas aeruginosa, Legionella and pathogenic staphylococci using standard microbiological techniques and culture method. The sixteen thermal springs mainly from the eastern part of Algeria, whose water temperature ranges between 39 and 93 \(^{\circ }\hbox {C}\) , were also subjected to hydrochemical and gamma spectrometric methods. The obtained results showed the average number of total aerobic flora ranging from 0 to \(4\times 10^{4}\) CFU/mL and the presence of Staphylococcus aureus species. Four springs revealed the presence of Legionella. Two springs among the four also exhibited a significant contamination by enterococci. The conductivity ranged from 296 to 48,100 \(\upmu \) S/cm. The thermal waters are rich in minerals representing the evaporite deposits leaching with high temperatures and significant mineralization in both major and minor elements. Measured activities of \(^{40}\) K ranged from 360 to 6,330 mBq/L. For the \(^{238}\hbox {U}\) series, \(^{226}\hbox {Ra}\) activity concentrations varied from 8 to 6,110 mBq/L. Activity concentrations determined for \(^{137}\hbox {Cs}\) revealed that only one thermal spring contained this radionuclide with concentration of 95 mBq/L.
    Water Quality Exposure and Health 06/2014; 7(2). DOI:10.1007/s12403-014-0130-x · 0.91 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A strictly anaerobic, moderately thermophilic, halotolerant, rod-shaped bacterium, was isolated from a water sample of a Tunisian hot spring. The strain designated BELH25T was non motile with cells appearing singly or in pairs (0.4-0.6 µm x 2-6 µm). It grew at temperatures between 45°C and 70°C (optimum 55°C), pH between 6.2 and 8.0 (optimum 7.0) and NaCl concentration between 0 and 4 % (optimum 0-2.0 %). Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate and nitrite were not used as terminal electron acceptors. Strain BELH25T used cellobiose, fructose, galactose, glucose, maltose, mannose, saccharose, starch and yeast extract as electron donors. The main fermentation products from glucose metabolism were formate, acetate, ethanol and CO(2). The predominant cellular fatty acids were iso- C15:0, iso- C17:0 and anteiso-C15:0. The DNA G+C content was 37.2 mol %. Phylogenetic analysis of the small-subunit (SSU) ribosomal RNA (rRNA) gene sequence indicated that strain BELH25T had Caloramator viterbiensis and Fervidicella metallireducens as its closest relatives (identity of 92.2 and 92.1 % respectively) and that the strain was positioned approximatively equidistantly between the two genera. Based on phenotypic, phylogenetic and taxonomic characteristics, strain BELH25T is proposed as a novel species of a new genus within the order Clostridiales, family Clostridiaceae for which the name Fonticella tunisiensis is proposed. The type strain is BELH25T (= DSM 24455 T = JCM 17559T).
    International Journal of Systematic and Evolutionary Microbiology 09/2012; 63(Pt 6). DOI:10.1099/ijs.0.041947-0 · 2.51 Impact Factor
Show more