Article

Production of synthetic seed by desiccation and encapsulation

Purdue University Department of Horticulture 47907 West Lafayette Indiana; University of Delaware Department of Plant Science 19717 Newark Delaware; Rural Development Administration Agricultural Sciences Institute 440-707 Suwon Korea
In Vitro Cellular & Developmental Biology - Plant (Impact Factor: 1.14). 25(12):1167-1172. DOI: 10.1007/BF02621269

ABSTRACT Producing synthetic seed of carrot consists of coating in-vitro grown embryos with a synthetic seed coat such as Polyox WSR-N
750, drying under controlled conditions, and hardening to prevent precocious germination. Survival of such embryos declines
over time. Similar procedures have also been used with celery. Somatic embryos have several advantages compared to conventional
tissue culture which include proliferacy, singulation, and the development of bipolar structures. The factors which most limit
the use of synthetic seeds are the inability to use such procedures with economically important genotypes, lack of understanding
of the maturation of somatic embryos and poor conversion rates to greenhouse and/or field.

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